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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P19-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KGF Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human KGF (FGF-7). Anti-Human KGF (FGF-7)-specific antibody was purified by affinity chromatography employing an immobilized Human KGF (FGF-7) matrix. Sandwich ELISA: To detect Human KGF (FGF-7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human KGF (FGF-7) (500-P19Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human KGF (FGF-7). Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references A novel solid-phase site-specific PEGylation enhances the in vitro and in vivo biostabilty of recombinant human keratinocyte growth factor 1.
Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing.
Huang Z, Zhu G, Sun C, Zhang J, Zhang Y, Zhang Y, Ye C, Wang X, Ilghari D, Li X
PloS one 2012;7(5):e36423
PloS one 2012;7(5):e36423
Regulation of epithelial-mesenchymal IL-1 signaling by PPARbeta/delta is essential for skin homeostasis and wound healing.
Chong HC, Tan MJ, Philippe V, Tan SH, Tan CK, Ku CW, Goh YY, Wahli W, Michalik L, Tan NS
The Journal of cell biology 2009 Mar 23;184(6):817-31
The Journal of cell biology 2009 Mar 23;184(6):817-31
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis KGF Polyclonal Antibody (Product # 500-P19-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human KGF (FGF-7) by Western Blot analysis KGF Polyclonal Antibody (Product # 500-P19-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human KGF (FGF-7) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human KGF (FGF-7) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of KGF Polyclonal Antibody (Product # 500-P19-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech KGF Polyclonal Antibody, Biotin (Product # 500-P19BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human KGF (FGF-7).
