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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- AGR-069-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR139 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-GPR139 Antibody (#AGR-069), (1:200). 2. Anti-GPR139 Antibody , preincubated with GPR139 Blocking Peptide (BLP-GR069).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): - 1-2. Anti-GPR139 Antibody (#AGR-069), (1:800). 3-4. Anti-GPR139 Antibody , preincubated with GPR139 Blocking Peptide (BLP-GR069).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR139 in rat ventromedial hypothalamus (VMH). Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR139 Antibody (#AGR-069), (1:600), followed by goat Anti-rabbit-AlexaFluor-488. A. GPR139 immunoreactivity (green) appears in tanycyte-like processes (arrows). B. Pre-incubation of the Antibody with GPR139 Blocking Peptide (BLP-GR069), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR139 in rat ventromedial hypothalamus (VMH). Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR139 Antibody (#AGR-069), (1:600), followed by goat Anti-rabbit-AlexaFluor-488. A. GPR139 immunoreactivity (green) appears in tanycyte-like processes (arrows). B. Pre-incubation of the Antibody with GPR139 Blocking Peptide (BLP-GR069), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = third ventricle.
