Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX118603 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX118603, RRID:AB_10617263
- Product name
- STRAP antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- STRAP antibody [N1C3] detects STRAP protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract10% SDS-PAGESTRAP antibody [N1C3] (GTX118603) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- STRAP antibody [N1C3] detects STRAP protein by western blot analysis.A. 30 ?g MCF-7 whole cell lysate/extractB. 30 ?g MDA-MB-231 whole cell lysate/extract 10% SDS-PAGESTRAP antibody [N1C3] (GTX118603) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
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- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (GTX118603) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with STRAP antibody [N1C3] (GTX118603) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- STRAP antibody [N1C3] detects STRAP protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: STRAP protein stained by STRAP antibody [N1C3] (GTX118603) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- STRAP antibody [N1C3] detects STRAP protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. STRAP antibody [N1C3] (GTX118603) dilution: 1:500.