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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- V7445 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MyoD1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G affinity chromatography
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 5.2F
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml, 1 mg/ml (BSA/azide free format), Prediluted (IHC only format)
- Storage
- Store the MyoD1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Submitted references Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation.
Premature senescence in primary muscle cultures of myotonic dystrophy type 2 is not associated with p16 induction.
Clinicopathological and extensive immunohistochemical study of a type II pleuropulmonary blastoma.
Inflammatory monomorphic undifferentiated sarcoma with distinct clinical and pathological features: a 'new' entity?
Dedifferentiated solitary fibrous tumour of the nasal cavity: the first case reported with molecular characterization of a TP53 mutation.
Elevated satellite cell number in Duchenne muscular dystrophy.
Culturing of diagnostic muscle biopsies as spheroid-like structures: a pilot study of morphology and viability.
Molecular characterization of dedifferentiated mucoepidermoid carcinoma of the trachea using laser microdissection-based TP53 mutation analysis.
Galactosylceramide expression factor-1 induces myogenesis in MDCK and C3H10T1/2 cells.
MyoD and myogenin protein expression in skeletal muscles of senile rats.
Alveolar soft-part sarcoma: a review of the pathology and histogenesis.
Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility.
Miersch C, Stange K, Röntgen M
BMC cell biology 2018 Mar 9;19(1):2
BMC cell biology 2018 Mar 9;19(1):2
Premature senescence in primary muscle cultures of myotonic dystrophy type 2 is not associated with p16 induction.
Renna LV, Cardani R, Botta A, Rossi G, Fossati B, Costa E, Meola G
European journal of histochemistry : EJH 2014 Oct 22;58(4):2444
European journal of histochemistry : EJH 2014 Oct 22;58(4):2444
Clinicopathological and extensive immunohistochemical study of a type II pleuropulmonary blastoma.
Yu L, Cheng H, Yang SJ
Fetal and pediatric pathology 2014 Feb;33(1):1-8
Fetal and pediatric pathology 2014 Feb;33(1):1-8
Inflammatory monomorphic undifferentiated sarcoma with distinct clinical and pathological features: a 'new' entity?
Wang Z, Huang G, Yan Q, Wang L, Zhu J, Lu Y, Li P, Cheng H, Ma MJ, Walker BF, Allen PW
Pathology 2011 Jan;43(1):48-53
Pathology 2011 Jan;43(1):48-53
Dedifferentiated solitary fibrous tumour of the nasal cavity: the first case reported with molecular characterization of a TP53 mutation.
Subramaniam MM, Lim XY, Venkateswaran K, Shuen CS, Soong R, Petersson F
Histopathology 2011 Dec;59(6):1269-74
Histopathology 2011 Dec;59(6):1269-74
Elevated satellite cell number in Duchenne muscular dystrophy.
Kottlors M, Kirschner J
Cell and tissue research 2010 Jun;340(3):541-8
Cell and tissue research 2010 Jun;340(3):541-8
Culturing of diagnostic muscle biopsies as spheroid-like structures: a pilot study of morphology and viability.
Gáti I, Danielsson O, Betmark T, Ernerudh J, Ollinger K, Dizdar N
Neurological research 2010 Jul;32(6):650-5
Neurological research 2010 Jul;32(6):650-5
Molecular characterization of dedifferentiated mucoepidermoid carcinoma of the trachea using laser microdissection-based TP53 mutation analysis.
Subramaniam MM, Ng SB, Seah SB, Anuar D, Soong R, Lee VK
Histopathology 2009 Oct;55(4):472-5
Histopathology 2009 Oct;55(4):472-5
Galactosylceramide expression factor-1 induces myogenesis in MDCK and C3H10T1/2 cells.
Ogura K, Niino YS, Tai T
Archives of biochemistry and biophysics 2004 Jun 15;426(2):279-85
Archives of biochemistry and biophysics 2004 Jun 15;426(2):279-85
MyoD and myogenin protein expression in skeletal muscles of senile rats.
Dedkov EI, Kostrominova TY, Borisov AB, Carlson BM
Cell and tissue research 2003 Mar;311(3):401-16
Cell and tissue research 2003 Mar;311(3):401-16
Alveolar soft-part sarcoma: a review of the pathology and histogenesis.
Ordóñez NG, Mackay B
Ultrastructural pathology 1998 Jul-Aug;22(4):275-92
Ultrastructural pathology 1998 Jul-Aug;22(4):275-92
Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility.
Dias P, Parham DM, Shapiro DN, Tapscott SJ, Houghton PJ
Cancer research 1992 Dec 1;52(23):6431-9
Cancer research 1992 Dec 1;52(23):6431-9
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human Rhabdomyosarcoma with MyoD1 antibody (clone 5.2F). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human skeletal muscle with MyoD1 antibody (clone 5.2F). HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free MyoD1 antibody (clone 5.2F) as confirmation of integrity and purity.
