MAB0077

antibody from Abnova Corporation

Targeting: MYOD1 bHLHc1, MYF3, MYOD, PUM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB0077

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB0077, RRID:AB_1019017

Product name

Myod1 monoclonal antibody, clone 5.8A

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant Myod1.

Isotype

IgG

Antibody clone number

5.8A

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Skeletal muscle insulin resistance induced by adipocyte-conditioned medium: underlying mechanisms and reversibility.

Sell H, Eckardt K, Taube A, Tews D, Gurgui M, Van Echten-Deckert G, Eckel J
American journal of physiology. Endocrinology and metabolism 2008 Jun;294(6):E1070-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis for Myod1 expression in various small round cell tumor lines using 1 ug/mL Myod1 monoclonal antibody, clone 5.8A (Cat # MAB0077) monoclonal antibody. The antibody only reacts with a band of approx. 45 kD in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry (Frozen sections) using Myod1 monoclonal antibody, clone 5.8A (Cat # MAB0077) monoclonal antibody. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent) .

Validation comment

Immunohistochemistry (Frozen sections)