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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-19568 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPY Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat mediated antigen retrieval recommended prior to tissue staining.
- Concentration
- 0.9 mg/mL
Submitted references SerpinA3N is a novel hypothalamic gene upregulated by a high-fat diet and leptin in mice.
Sergi D, Campbell FM, Grant C, Morris AC, Bachmair EM, Koch C, McLean FH, Muller A, Hoggard N, de Roos B, Porteiro B, Boekschoten MV, McGillicuddy FC, Kahn D, Nicol P, Benzler J, Mayer CD, Drew JE, Roche HM, Muller M, Nogueiras R, Dieguez C, Tups A, Williams LM
Genes & nutrition 2018;13:28
Genes & nutrition 2018;13:28
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human cerebral cortex tissue using Product # PA5-19568, anti-Neuropeptide Y antibody. Primary antibody was used at a concentration of 1 µg/mL and exposed for 8 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6/20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 a Representative in situ autoradiograph of a C57BL/6J mouse brain section after 4 weeks of HFD showing serpinA3N expression in the arcuate nuclei (ARC), the ventromedial nuclei of the hypothalamus (VMH), the dorsomedial nuclei of the hypothalamus (DMH) and the lateral hypothalamus (LH). b - e Representative immunostaining of alpha 1 AC (brown) and b GFAP, c Iba1, d AgRP and e NPY (all blue) in the arcuate nuclei after 1 week of HFD. Bar = 20 mum
