12345-1-AP
antibody from Invitrogen Antibodies
Targeting: DYNC1H1
CMT2O, DHC1, DNCH1, Dnchc1, DNCL, DNECL, HL-3, p22
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- 12345-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DYNC1H1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAQSIYGGR VDNEFDQRLL NTFLERLFTT RSFDSEFKLA CKVDGHKDIQ MPDGIRREEF VQWVELLPDT QTPSWLGLPN NAERVLLTTQ GVDMISKMLK MQMLEDEDDL AYAETEKKTR TDSTSDGRPA WMRTLHTTAS NWLHLIPQTL SHLKRTVENI KDPLFRFFER EVKMGAKLLQ DVRQDLADVV QVCEGKKKQT NYLRTLINEL VKGILPRSWS HYTVPAGMTV IQWVSDFSER IKQLQNISLA AASGGAKELK NIHVCLGGLF VPEAYITATR QYVAQANSWS LEELCLEVNV TTSQGATLDA CSFGVTGLKL QGATCNNNKL SLSNAISTAL PLTQLRWVKQ TNTEKKASVV TLPVYLNFTR ADLIFTVDFE IATKEDPRSF YERGVAVLCT E (590-989 aa encoded by BC021297)
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12345-1-AP (DYNC1H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using DYNC1H1 antibody 12345-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis slide using 12345-1-AP (DYNC1H1 antibody at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 12345-1-AP (DYNC1H1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).