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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-10729 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-10729, RRID:AB_647474
- Product name
- Anti-ITGA5
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Acheron, a Lupus antigen family member, regulates integrin expression, adhesion, and motility in differentiating myoblasts.
A chemical biology screen reveals a role for Rab21-mediated control of actomyosin contractility in fibroblast-driven cancer invasion.
Interaction of monocarboxylate transporter 4 with beta1-integrin and its role in cell migration.
Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells
Arginine-glycine-aspartic acid (RGD)-containing peptides inhibit the force production of mouse papillary muscle bundles via alpha 5 beta 1 integrin.
Massive hepatic apoptosis associated with TGF-beta1 activation after Fas ligand treatment of IGF binding protein-1-deficient mice.
Glenn HL, Wang Z, Schwartz LM
American journal of physiology. Cell physiology 2010 Jan;298(1):C46-55
American journal of physiology. Cell physiology 2010 Jan;298(1):C46-55
A chemical biology screen reveals a role for Rab21-mediated control of actomyosin contractility in fibroblast-driven cancer invasion.
Hooper S, Gaggioli C, Sahai E
British journal of cancer 2010 Jan 19;102(2):392-402
British journal of cancer 2010 Jan 19;102(2):392-402
Interaction of monocarboxylate transporter 4 with beta1-integrin and its role in cell migration.
Gallagher SM, Castorino JJ, Philp NJ
American journal of physiology. Cell physiology 2009 Mar;296(3):C414-21
American journal of physiology. Cell physiology 2009 Mar;296(3):C414-21
Fibroblast-led collective invasion of carcinoma cells with differing roles for RhoGTPases in leading and following cells
Cedric Gaggioli, Steven Hooper, Cristina Hidalgo-Carcedo, Robert Grosse, John F. Marshall, Kevin Harrington, Erik Sahai
Nature Cell Biology 2007 Nov;9(12):1392-1400
Nature Cell Biology 2007 Nov;9(12):1392-1400
Arginine-glycine-aspartic acid (RGD)-containing peptides inhibit the force production of mouse papillary muscle bundles via alpha 5 beta 1 integrin.
Sarin V, Gaffin RD, Meininger GA, Muthuchamy M
The Journal of physiology 2005 Apr 15;564(Pt 2):603-17
The Journal of physiology 2005 Apr 15;564(Pt 2):603-17
Massive hepatic apoptosis associated with TGF-beta1 activation after Fas ligand treatment of IGF binding protein-1-deficient mice.
Leu JI, Crissey MA, Taub R
The Journal of clinical investigation 2003 Jan;111(1):129-39
The Journal of clinical investigation 2003 Jan;111(1):129-39
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 229, 107, 78, 46, 31, 26, 16.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 115
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human placenta shows distinct cytoplasmic positivity in trophoblastic cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
