Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF1864 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Integrin alpha 5/CD49e Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin alpha 5 and less than 5% cross-reactivity with recombinant human Integrin alpha M, rmIntegrin alpha 2, rmIntegrin alpha 4, and rmIntegrin alpha E is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08648- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Macrophage-secreted factors promote a profibrotic phenotype in human preadipocytes.
Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.
Keophiphath M, Achard V, Henegar C, Rouault C, Clément K, Lacasa D
Molecular endocrinology (Baltimore, Md.) 2009 Jan;23(1):11-24
Molecular endocrinology (Baltimore, Md.) 2009 Jan;23(1):11-24
Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.
Page JL, Johnson MC, Olsavsky KM, Strom SC, Zarbl H, Omiecinski CJ
Toxicological sciences : an official journal of the Society of Toxicology 2007 Jun;97(2):384-97
Toxicological sciences : an official journal of the Society of Toxicology 2007 Jun;97(2):384-97
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Integrin a5/CD49e by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a5/CD49e at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
