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- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF3856 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Serum Amyloid A4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Serum Amyloid A4 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Serum Amyloid A4 is observed and less than 5% cross-reactivity with recombinant human (rh) Serum Amyloid A1 and rhSerum Amyloid A2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A proteomic approach identifies early pregnancy biomarkers for preeclampsia: novel linkages between a predisposition to preeclampsia and cardiovascular disease.
Blumenstein M, McMaster MT, Black MA, Wu S, Prakash R, Cooney J, McCowan LM, Cooper GJ, North RA
Proteomics 2009 Jun;9(11):2929-45
Proteomics 2009 Jun;9(11):2929-45
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Serum Amyloid A4 in Human Liver. Serum Amyloid A4 was detected in immersion fixed paraffin-embedded sections of human liver using 15 µg/mL Goat Anti-Human Serum Amyloid A4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3856) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
