GTX110543

antibody from GeneTex

Targeting: CASP3 apopain, CPP32, CPP32B, Yama

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX110543

Provider

GeneTex

Proper citation

GeneTex Cat#GTX110543, RRID:AB_10722709

Product name

Caspase 3 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Monoamine oxidase-A promotes protective autophagy in human SH-SY5Y neuroblastoma cells through Bcl-2 phosphorylation.

Ugun-Klusek A, Theodosi TS, Fitzgerald JC, Burté F, Ufer C, Boocock DJ, Yu-Wai-Man P, Bedford L, Billett EE
Redox biology 2019 Jan;20:167-181

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Endoplasmic reticulum-targeting doxorubicin: a new tool effective against doxorubicin-resistant osteosarcoma.

Buondonno I, Gazzano E, Tavanti E, Chegaev K, Kopecka J, Fanelli M, Rolando B, Fruttero R, Gasco A, Hattinger C, Serra M, Riganti C
Cellular and molecular life sciences : CMLS 2019 Feb;76(3):609-625

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An augmentation in histone dimethylation at lysine nine residues elicits vision impairment following traumatic brain injury.

Gupta R, Saha P, Sen T, Sen N
Free radical biology & medicine 2019 Feb 18;134:630-643

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Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.

Lee CW, Chi MC, Chang TM, Liu JF
Journal of cellular physiology 2019 Aug;234(8):13157-13168

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Rhodiola crenulata Attenuates γ-Ray Induced Cellular Injury via Modulation of Oxidative Stress in Human Skin Cells.

Lin KT, Chang TC, Lai FY, Lin CS, Chao HL, Lee SY
The American journal of Chinese medicine 2018;46(1):175-190

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Benzyl isothiocyanate (BITC) triggers mitochondria-mediated apoptotic machinery in human cisplatin-resistant oral cancer CAR cells.

Lee CF, Chiang NN, Lu YH, Huang YS, Yang JS, Tsai SC, Lu CC, Chen FA
BioMedicine 2018 Sep;8(3):15

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Resveratrol inhibits human leiomyoma cell proliferation via crosstalk between integrin αvβ3 and IGF-1R.

Ho Y, Sh Yang YC, Chin YT, Chou SY, Chen YR, Shih YJ, Whang-Peng J, Changou CA, Liu HL, Lin SJ, Tang HY, Lin HY, Davis PJ
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Oct;120:346-355

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GanDouLing combined with Penicillamine improves cerebrovascular injury via PERK/eIF2α/CHOP endoplasmic reticulum stress pathway in the mouse model of Wilson's disease.

Chen Y, Zhang B, Cao S, Huang W, Liu N, Yang W
Bioscience reports 2018 Oct 31;38(5)

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A high-throughput pipeline for validation of antibodies.

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912

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Pterostilbene modulates the suppression of multidrug resistance protein 1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling.

Chang HP, Lu CC, Chiang JH, Tsai FJ, Juan YN, Tsao JW, Chiu HY, Yang JS
International journal of oncology 2018 May;52(5):1504-1514

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Effects of ERK1/2 S-nitrosylation on ERK1/2 phosphorylation and cell survival in glioma cells.

Jin L, Cao Y, Zhang T, Wang P, Ji D, Liu X, Shi H, Hua L, Yu R, Gao S
International journal of molecular medicine 2018 Mar;41(3):1339-1348

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MicroRNA-630 inhibitor sensitizes chemoresistant ovarian cancer to chemotherapy by enhancing apoptosis.

Eoh KJ, Lee SH, Kim HJ, Lee JY, Kim S, Kim SW, Kim YT, Nam EJ
Biochemical and biophysical research communications 2018 Mar 4;497(2):513-520

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ITR‑284 modulates cell differentiation in human chronic myelogenous leukemia K562 cells.

Yang JS, Lee CY, Cho HC, Lu CC, Kuo SC, Wen YF, Tsai FJ, Lee MR, Tsai SC
Oncology reports 2018 Jan;39(1):383-391

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Ubiquitination of the Cytoplasmic Domain of Influenza A Virus M2 Protein Is Crucial for Production of Infectious Virus Particles.

Su WC, Yu WY, Huang SH, Lai MMC
Journal of virology 2018 Feb 15;92(4)

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A new copper ionophore DPMQ protects cells against ultraviolet B irradiation by inhibiting the TRPV1 channel.

Huang KF, Ma KH, Hung YC, Lo LC, Lin KC, Liu PS, Hu MK, Chueh SH
Journal of cellular physiology 2018 Dec;233(12):9594-9610

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Escin induces apoptosis in human bladder cancer cells: An in vitro and in vivo study.

Cheng CL, Chao WT, Li YH, Ou YC, Wang SS, Chiu KY, Yuan SY
European journal of pharmacology 2018 Dec 5;840:79-88

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Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.

Terasaki M, Ogawa Y, Endo T, Maeda H, Hamada J, Osada K, Miyashita K, Mutoh M
Anticancer research 2018 Apr;38(4):2169-2179

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Response of Myeloid Leukemia Cells to Luteolin is Modulated by Differentially Expressed Pituitary Tumor-Transforming Gene 1 (PTTG1) Oncoprotein.

Chen PY, Tien HJ, Chen SF, Horng CT, Tang HL, Jung HL, Wu MJ, Yen JH
International journal of molecular sciences 2018 Apr 12;19(4)

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Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes.

Zera K, Zastre J
PloS one 2017;12(10):e0186707

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Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma.

Li SM, Chen CH, Chen YW, Yen YC, Fang WT, Tsai FY, Chang JL, Shen YY, Huang SF, Chuu CP, Chang IS, Hsiung CA, Jiang SS
Scientific reports 2017 Sep 19;7(1):11893

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High-glucose induces tau hyperphosphorylation through activation of TLR9-P38MAPK pathway.

Sun Y, Xiao Q, Luo C, Zhao Y, Pu D, Zhao K, Chen J, Wang M, Liao Z
Experimental cell research 2017 Oct 15;359(2):312-318

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Effect of Some Growth Factors on Tissue Transglutaminase Overexpression Induced by β-Amyloid in Olfactory Ensheathing Cells.

Pellitteri R, Bonfanti R, Spatuzza M, Cambria MT, Ferrara M, Raciti G, Campisi A
Molecular neurobiology 2017 Nov;54(9):6785-6794

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Panobinostat sensitizes KRAS-mutant non-small-cell lung cancer to gefitinib by targeting TAZ.

Lee WY, Chen PC, Wu WS, Wu HC, Lan CH, Huang YH, Cheng CH, Chen KC, Lin CW
International journal of cancer 2017 Nov 1;141(9):1921-1931

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Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model.

Li J, Luo H, Dong X, Liu Q, Wu C, Zhang T, Hu X, Zhang Y, Song B, Li L
Stem cell research & therapy 2017 May 8;8(1):107

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Functional and biochemical characterization of a T cell-associated anti-apoptotic protein, GIMAP6.

Ho CH, Tsai SF
The Journal of biological chemistry 2017 Jun 2;292(22):9305-9319

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Extracellular signal-regulated kinase 8-mediated NF-κB activation increases sensitivity of human lung cancer cells to arsenic trioxide.

Wu DD, Lau ATY, Yu FY, Cai NL, Dai LJ, Ok Kim M, Jin DY, Xu YM
Oncotarget 2017 Jul 25;8(30):49144-49155

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Rhodiola crenulata extract counteracts the effect of hypobaric hypoxia in rat heart via redirection of the nitric oxide and arginase 1 pathway.

Hsu SW, Chang TC, Wu YK, Lin KT, Shi LS, Lee SY
BMC complementary and alternative medicine 2017 Jan 7;17(1):29

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Differential resistance to platinum-based drugs and 5-fluorouracil in p22phox-overexpressing oral squamous cell carcinoma: Implications of alternative treatment strategies.

Hung CC, Chien CY, Chu PY, Wu YJ, Lin CS, Huang CJ, Chan LP, Wang YY, Yuan SF, Hour TC, Chen JY
Head & neck 2017 Aug;39(8):1621-1630

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Hydroxytyrosol Induces Apoptosis and Cell Cycle Arrest and Suppresses Multiple Oncogenic Signaling Pathways in Prostate Cancer Cells.

Zubair H, Bhardwaj A, Ahmad A, Srivastava SK, Khan MA, Patel GK, Singh S, Singh AP
Nutrition and cancer 2017 Aug-Sep;69(6):932-942

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4β-Hydroxywithanolide E Modulates Alternative Splicing of Apoptotic Genes in Human Hepatocellular Carcinoma Huh-7 Cells.

Lee CC, Chang WH, Chang YS, Liu TY, Chen YC, Wu YC, Chang JG
Scientific reports 2017 Aug 4;7(1):7290

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Galangin ameliorates cisplatin-induced nephrotoxicity by attenuating oxidative stress, inflammation and cell death in mice through inhibition of ERK and NF-kappaB signaling.

Huang YC, Tsai MS, Hsieh PC, Shih JH, Wang TS, Wang YC, Lin TH, Wang SH
Toxicology and applied pharmacology 2017 Aug 15;329:128-139

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The CHAC1-inhibited Notch3 pathway is involved in temozolomide-induced glioma cytotoxicity.

Chen PH, Shen WL, Shih CM, Ho KH, Cheng CH, Lin CW, Lee CC, Liu AJ, Chen KC
Neuropharmacology 2017 Apr;116:300-314

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Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells.

Lin CY, Chang TW, Hsieh WH, Hung MC, Lin IH, Lai SC, Tzeng YJ
Cell death discovery 2016;2:16065

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Photodynamic-therapy Activates Immune Response by disrupting Immunity Homeostasis of Tumor Cells, which Generates Vaccine for Cancer Therapy.

Zheng Y, Yin G, Le V, Zhang A, Chen S, Liang X, Liu J
International journal of biological sciences 2016;12(1):120-32

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Pigment Epithelium-Derived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury.

Kuo HF, Liu PL, Chong IW, Liu YP, Chen YH, Ku PM, Li CY, Chen HH, Chiang HC, Wang CL, Chen HJ, Chen YC, Hsieh CC
PloS one 2016;11(5):e0156059

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The aurora kinase inhibitor VX-680 shows anti-cancer effects in primary metastatic cells and the SW13 cell line.

Pezzani R, Rubin B, Bertazza L, Redaelli M, Barollo S, Monticelli H, Baldini E, Mian C, Mucignat C, Scaroni C, Mantero F, Ulisse S, Iacobone M, Boscaro M
Investigational new drugs 2016 Oct;34(5):531-40

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MGDG extracted from spinach enhances the cytotoxicity of radiation in pancreatic cancer cells.

Akasaka H, Mizushina Y, Yoshida K, Ejima Y, Mukumoto N, Wang T, Inubushi S, Nakayama M, Wakahara Y, Sasaki R
Radiation oncology (London, England) 2016 Nov 22;11(1):153

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Effects of Oxymatrine on the Proliferation and Apoptosis of Human Hepatoma Carcinoma Cells.

Liu Y, Bi T, Dai W, Wang G, Qian L, Gao Q, Shen G
Technology in cancer research & treatment 2016 Jun;15(3):487-97

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Cabozantinib is selectively cytotoxic in acute myeloid leukemia cells with FLT3-internal tandem duplication (FLT3-ITD).

Lu JW, Wang AN, Liao HA, Chen CY, Hou HA, Hu CY, Tien HF, Ou DL, Lin LI
Cancer letters 2016 Jul 1;376(2):218-25

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Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.

Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT
Oncotarget 2016 Feb 2;7(5):6146-58

---

Chronic Hypobaric Hypoxia Induces Right Ventricular Hypertrophy and Apoptosis in Rats: Therapeutic Potential of Nanocurcumin in Improving Adaptation.

Nehra S, Bhardwaj V, Kar S, Saraswat D
High altitude medicine & biology 2016 Dec;17(4):342-352

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Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.

Lee WY, Lee WT, Cheng CH, Chen KC, Chou CM, Chung CH, Sun MS, Cheng HW, Ho MN, Lin CW
Oncotarget 2015 Sep 29;6(29):27580-95

---

Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.

Lee WY, Lee WT, Cheng CH, Chen KC, Chou CM, Chung CH, Sun MS, Cheng HW, Ho MN, Lin CW
Oncotarget 2015 Sep 29;6(29):27580-95

---

Autophagy inhibition enhances isorhamnetin‑induced mitochondria‑dependent apoptosis in non‑small cell lung cancer cells.

Ruan Y, Hu K, Chen H
Molecular medicine reports 2015 Oct;12(4):5796-806

---

The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.

Chou SF, Tsai ML, Huang JY, Chang YS, Shih C
PLoS pathogens 2015 Oct;11(10):e1005123

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p22phox confers resistance to cisplatin, by blocking its entry into the nucleus.

Hung CC, Chien CY, Chiang WF, Lin CS, Hour TC, Chen HR, Wang LF, Ko JY, Chang CH, Chen JY
Oncotarget 2015 Feb 28;6(6):4110-25

---

Inhibition of chemokine (C-C motif) receptor 7 sialylation suppresses CCL19-stimulated proliferation, invasion and anti-anoikis.

Su ML, Chang TM, Chiang CH, Chang HC, Hou MF, Li WS, Hung WC
PloS one 2014;9(6):e98823

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Chemical composition, antimicrobial activity against Staphylococcus aureus and a pro-apoptotic effect in SGC-7901 of the essential oil from Toona sinensis (A. Juss.) Roem. leaves.

Wu JG, Peng W, Yi J, Wu YB, Chen TQ, Wong KH, Wu JZ
Journal of ethnopharmacology 2014 May 28;154(1):198-205

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Induction of ROS-independent JNK-activation-mediated apoptosis by a novel coumarin-derivative, DMAC, in human colon cancer cells.

Lin MH, Cheng CH, Chen KC, Lee WT, Wang YF, Xiao CQ, Lin CW
Chemico-biological interactions 2014 Jul 25;218:42-9

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Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.

Li KC, Hua KT, Lin YS, Su CY, Ko JY, Hsiao M, Kuo ML, Tan CT
Molecular cancer 2014 Jul 15;13:172

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Tumor necrosis factor suppresses NR5A2 activity and intestinal glucocorticoid synthesis to sustain chronic colitis.

Huang SC, Lee CT, Chung BC
Science signaling 2014 Feb 25;7(314):ra20

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MicroRNA-302b-inhibited E2F3 transcription factor is related to all trans retinoic acid-induced glioma cell apoptosis.

Chen PH, Shih CM, Chang WC, Cheng CH, Lin CW, Ho KH, Su PC, Chen KC
Journal of neurochemistry 2014 Dec;131(6):731-42

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Anti-proliferative and gene expression actions of resveratrol in breast cancer cells in vitro.

Chin YT, Hsieh MT, Yang SH, Tsai PW, Wang SH, Wang CC, Lee YS, Cheng GY, HuangFu WC, London D, Tang HY, Fu E, Yen Y, Liu LF, Lin HY, Davis PJ
Oncotarget 2014 Dec 30;5(24):12891-907

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An extract of Rhodobacter sphaeroides reduces cisplatin-induced nephrotoxicity in mice.

Chang WW, Liu JJ, Liu CF, Liu WS, Lim YP, Cheng YJ, Lee CH
Toxins 2013 Nov 29;5(12):2353-65

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

42214

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate)      A: NIH-3T3     B: BCL-1     C: C2C12     D: Raw264.7     12% SDS PAGE      GTX110543 diluted at 1:1000      

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 12% SDS PAGE GTX110543 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HeLa treated with 0uM cisplatin for 24hr B: HeLa treated with 30uM cisplatin for 24hr 12% SDS PAGE GTX110543 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Caspase 3 antibody detects Caspase 3 protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extractB. 30 ?g Raji whole cell lysate/extractC. 30 ?g NCI-H929 whole cell lysate/extract 12% SDS-PAGECaspase 3 antibody (GTX110543) dilution: 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Caspase 3 antibody detects Caspase 3 protein by western blot analysis.A. 30 ?g K562 whole cell lysate/extract B. 30 ?g THP-1 whole cell lysate/extractC. 30 ?g HL-60 whole cell lysate/extract12% SDS-PAGECaspase 3 antibody (GTX110543) dilution: 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Caspase 3 antibody detects Caspase 3 protein by western blot analysis.A. 30 ?g HeLa whole cell lysate/extractB. 30 ?g HeLa whole cell lysate/extract (30 ?M cisplatin treatment for 24 hr) C. 30 ?g HeLa whole cell lysate/extract (30 ?M Cisplatin treatment for 48 hr) 12 % SDS-PAGECaspase 3 antibody (GTX110543) dilution: 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Caspase 3 antibody detects Caspase 3 protein by western blot analysis.A. 30 ?g C8D30 whole cell extractB. 30 ?g NIH-3T3 whole cell extractC. 30 ?g Raw264.7 whole cell extractD. 30 ?g C2C12 whole cell extract12% SDS-PAGECaspase 3 antibody (GTX110543) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Caspase 3 antibody detects Caspase 3 protein by western blot analysis. Un-treated (-) and treated (+, 30 ?M Cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody (GTX110543) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Caspase 3 antibody detects CASP3 protein at Cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CASP3 protein stained by Caspase 3 antibody (GTX110543) diluted at 1:500.Blue: Hoechst 33343 staining.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of Caspase 3 protein from HeLa whole cell extracts using 5 £gg of Caspase 3 antibody (GTX110543).Western blot analysis was performed using Caspase 3 antibody (GTX110543).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Caspase 3(GTX110543) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Caspase 3 antibody detects Caspase 3 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Caspase 3 antibody (GTX110543) diluted at 1:500.