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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA300422 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA300422, RRID:AB_2228482
- Product name
- Rabbit Monoclonal Antibody against CASP3 (Clone E83-103)
- Antibody type
- Monoclonal
- Description
- Rabbit Monoclonal Antibody against CASP3 (Clone E83-103)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CASP3
- Isotype
- IgG
- Antibody clone number
- E83-103
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - pro Caspase 3 antibody [Y83-103]; All lanes : Anti-pro Caspase 3 antibody [E83-103] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate + Camptothecin .Predicted band size : 31 kDa.Observed band size : 35 kDa .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ICC/IF image of TA300422 stained HeLa cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin-embedded sections) - pro Caspase 3 antibody [Y83-103]; Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma TA300422 at 1/250 dilution.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry-Anti-pro Caspase 3 antibody(TA300422); Overlay histogram showing Jurkat cells stained with TA300422 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
- Validation comment
- FC
