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Learn200-301-H63
antibody from Rockland Immunochemicals, Inc.
Targeting: CASP3
apopain, CPP32, CPP32B, Yama
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- 200-301-H63 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-H63, RRID:AB_2611282
- Product name
- Anti-Caspase-3 (MOUSE) Monoclonal Antibody - 200-301-H63
- Antibody type
- Monoclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Immunohistochemistry of mouse Anti-Caspase-3 antibody. Tissue: human stomach (top image/high magnification). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Caspase-3 at 5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-3 is cytoplasmic in some cells and nuclear in others. Caspase-3 in the nucleus is considered to be an indication of active caspase 3. In most cell types and model systems, cells with active caspase-3 are undergoing apoptosis. Staining: Caspase-3 has a DAB chromogen and Hematoxylin counterstain.
- Validation comment
- Immunohistochemistry
