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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB2069 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2069, RRID:AB_1572136
- Product name
- CASP3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CASP3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Radiation enhances caspase 3 cleavage of Rad51 in BRCA2-defective cells.
Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a.
Differential expression of mycobacterial antigen MPT64, apoptosis and inflammatory markers in multinucleated giant cells and epithelioid cells in granulomas caused by Mycobacterium tuberculosis.
Brown ET, Robinson-Benion C, Holt JT
Radiation research 2008 May;169(5):595-601
Radiation research 2008 May;169(5):595-601
Identification of caspase 3 motifs and critical aspartate residues in human phospholipase D1b and phospholipase D2a.
Wright MH, Farquhar MJ, Aletrari MO, Ladds G, Hodgkin MN
Biochemical and biophysical research communications 2008 May 2;369(2):478-84
Biochemical and biophysical research communications 2008 May 2;369(2):478-84
Differential expression of mycobacterial antigen MPT64, apoptosis and inflammatory markers in multinucleated giant cells and epithelioid cells in granulomas caused by Mycobacterium tuberculosis.
Mustafa T, Wiker HG, Mørkve O, Sviland L
Virchows Archiv : an international journal of pathology 2008 Apr;452(4):449-56
Virchows Archiv : an international journal of pathology 2008 Apr;452(4):449-56
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CASP3 polyclonal antibody (Cat # PAB2069) in NCI-H460 cell line lysates (35 ug/lane).CASP3 (arrow) was detected using the purified Polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CASP3 polyclonal antibody (Cat # PAB2069) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using CASP3 polyclonal antibody (Cat # PAB2069)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
