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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM08377PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM08377PU-N, RRID:AB_2035249
- Product name
- anti Caspase-3
- Antibody type
- Monoclonal
- Antigen
- Full-length recombinant Human Caspase-3.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 31A1067Â
- Vial size
- 50 µg
- Concentration
- 0.5 mg/ml
Submitted references Mutation in a primate-conserved retrotransposon reveals a noncoding RNA as a mediator of infantile encephalopathy.
Cartault F, Munier P, Benko E, Desguerre I, Hanein S, Boddaert N, Bandiera S, Vellayoudom J, Krejbich-Trotot P, Bintner M, Hoarau JJ, Girard M, Génin E, de Lonlay P, Fourmaintraux A, Naville M, Rodriguez D, Feingold J, Renouil M, Munnich A, Westhof E, Fähling M, Lyonnet S, Henrion-Caude A
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 27;109(13):4980-5
Proceedings of the National Academy of Sciences of the United States of America 2012 Mar 27;109(13):4980-5
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 in HeLa cells. The antibody detected both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Caspase-3 in multiple human tissues. The tissues shown are A) brain, B) heart, C) intestine, D) kidney, E) liver, F) lung, G) muscle, H) stomach, I) spleen, J) ovary, and K) testis. Lanes 1, 2 and 3 demonstrate the species crossreactivity of the antibody in Human, Mouse and Rat heart lysate, respectively.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AM08377PU-N Caspase-3 antibody staining of Formalin-Fixed Paraffin-Embedded Human Thymus at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
