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- Antibody Data
- Antigen structure
- References [40]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB835 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
U26943- Isotype
- IgG
- Antibody clone number
- 269518
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll‑like receptor‑4‑NADPH oxidase 4 signaling pathway.
The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination.
The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway.
Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function.
Expression of the neuroprotective protein aryl hydrocarbon receptor nuclear translocator 2 correlates with neuronal stress and disability in models of multiple sclerosis.
Lect2 deficiency is characterised by altered cytokine levels and promotion of intestinal tumourigenesis.
Trabecular meshwork morphogenesis: A comparative analysis of wildtype and anterior segment dysgenesis mouse models.
Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis.
Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo.
Formyl peptide receptor 1 suppresses gastric cancer angiogenesis and growth by exploiting inflammation resolution pathways.
Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells.
WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response.
Resveratrol fuels HER2 and ERα-positive breast cancer behaving as proteasome inhibitor.
H-Ras and K-Ras Oncoproteins Induce Different Tumor Spectra When Driven by the Same Regulatory Sequences.
Loss of Tmem30a leads to photoreceptor degeneration.
The protective effects of Resveratrol against radiation-induced intestinal injury.
Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration.
Melatonin alleviates lipopolysaccharide-compromised integrity of blood-brain barrier through activating AMP-activated protein kinase in old mice.
Potent Vasoconstrictor Kisspeptin-10 Induces Atherosclerotic Plaque Progression and Instability: Reversal by its Receptor GPR54 Antagonist.
Impaired Epidermal to Dendritic T Cell Signaling Slows Wound Repair in Aged Skin.
Chimeric Mice with Competent Hematopoietic Immunity Reproduce Key Features of Severe Lassa Fever.
Antitumor cytotoxicity induced by bone-marrow-derived antigen-presenting cells is facilitated by the tumor suppressor protein p53 via regulation of IL-12.
Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia.
The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
Edaravone protect against retinal damage in streptozotocin-induced diabetic mice.
Evaluation of antiviral efficacy of ribavirin, arbidol, and T-705 (favipiravir) in a mouse model for Crimean-Congo hemorrhagic fever.
Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-β.
Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-κB downregulation.
Proinflammatory cytokines differentially regulate adipocyte mitochondrial metabolism, oxidative stress, and dynamics.
The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.
Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
The ROS scavenger, NAC, regulates hepatic Vα14iNKT cells signaling during Fas mAb-dependent fulminant liver failure.
FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis.
Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
In-vivo transfection of pcDNA3.1-IGFBP7 inhibits melanoma growth in mice through apoptosis induction and VEGF downexpression.
Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury.
Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells.
You ZP, Yu MJ, Zhang YL, Shi K
Molecular medicine reports 2019 Jan;19(1):382-390
Molecular medicine reports 2019 Jan;19(1):382-390
The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination.
Zahednasab H, Firouzi M, Kaboudanian-Ardestani S, Mojallal-Tabatabaei Z, Karampour S, Keyvani H
Cytokine 2019 Jan;113:417-426
Cytokine 2019 Jan;113:417-426
The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway.
Shen P, Chen J, Pan M
Molecular medicine reports 2018 Sep;18(3):3332-3340
Molecular medicine reports 2018 Sep;18(3):3332-3340
Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function.
Hamon R, Tran HB, Roscioli E, Ween M, Jersmann H, Hodge S
Scientific reports 2018 Sep 7;8(1):13424
Scientific reports 2018 Sep 7;8(1):13424
Expression of the neuroprotective protein aryl hydrocarbon receptor nuclear translocator 2 correlates with neuronal stress and disability in models of multiple sclerosis.
Rahim T, Becquart P, Baeva ME, Quandt J
Journal of neuroinflammation 2018 Sep 19;15(1):270
Journal of neuroinflammation 2018 Sep 19;15(1):270
Lect2 deficiency is characterised by altered cytokine levels and promotion of intestinal tumourigenesis.
Greenow KR, Zverev M, May S, Kendrick H, Williams GT, Phesse T, Parry L
Oncotarget 2018 Nov 23;9(92):36430-36443
Oncotarget 2018 Nov 23;9(92):36430-36443
Trabecular meshwork morphogenesis: A comparative analysis of wildtype and anterior segment dysgenesis mouse models.
Rausch RL, Libby RT, Kiernan AE
Experimental eye research 2018 May;170:81-91
Experimental eye research 2018 May;170:81-91
Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis.
Moon BS, Bai J, Cai M, Liu C, Shi J, Lu W
Nature communications 2018 Jan 26;9(1):401
Nature communications 2018 Jan 26;9(1):401
Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo.
Shaalan A, Carpenter G, Proctor G
Molecular immunology 2018 Feb;94:190-199
Molecular immunology 2018 Feb;94:190-199
Formyl peptide receptor 1 suppresses gastric cancer angiogenesis and growth by exploiting inflammation resolution pathways.
Prevete N, Liotti F, Illiano A, Amoresano A, Pucci P, de Paulis A, Melillo RM
Oncoimmunology 2017;6(4):e1293213
Oncoimmunology 2017;6(4):e1293213
Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells.
Beer L, Nemec L, Wagner T, Ristl R, Altenburger LM, Ankersmit HJ, Mildner M
Journal of radiation research 2017 Mar 1;58(2):201-209
Journal of radiation research 2017 Mar 1;58(2):201-209
WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response.
Janczar S, Nautiyal J, Xiao Y, Curry E, Sun M, Zanini E, Paige AJ, Gabra H
Cell death & disease 2017 Jul 27;8(7):e2955
Cell death & disease 2017 Jul 27;8(7):e2955
Resveratrol fuels HER2 and ERα-positive breast cancer behaving as proteasome inhibitor.
Andreani C, Bartolacci C, Wijnant K, Crinelli R, Bianchi M, Magnani M, Hysi A, Iezzi M, Amici A, Marchini C
Aging 2017 Feb 26;9(2):508-523
Aging 2017 Feb 26;9(2):508-523
H-Ras and K-Ras Oncoproteins Induce Different Tumor Spectra When Driven by the Same Regulatory Sequences.
Drosten M, Simón-Carrasco L, Hernández-Porras I, Lechuga CG, Blasco MT, Jacob HK, Fabbiano S, Potenza N, Bustelo XR, Guerra C, Barbacid M
Cancer research 2017 Feb 1;77(3):707-718
Cancer research 2017 Feb 1;77(3):707-718
Loss of Tmem30a leads to photoreceptor degeneration.
Zhang L, Yang Y, Li S, Zhang S, Zhu X, Tai Z, Yang M, Liu Y, Guo X, Chen B, Jiang Z, Lu F, Zhu X
Scientific reports 2017 Aug 24;7(1):9296
Scientific reports 2017 Aug 24;7(1):9296
The protective effects of Resveratrol against radiation-induced intestinal injury.
Zhang H, Yan H, Zhou X, Wang H, Yang Y, Zhang J, Wang H
BMC complementary and alternative medicine 2017 Aug 16;17(1):410
BMC complementary and alternative medicine 2017 Aug 16;17(1):410
Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration.
Sallustio F, Curci C, Aloisi A, Toma CC, Marulli E, Serino G, Cox SN, De Palma G, Stasi A, Divella C, Rinaldi R, Schena FP
Scientific reports 2017 Aug 15;7(1):8225
Scientific reports 2017 Aug 15;7(1):8225
Melatonin alleviates lipopolysaccharide-compromised integrity of blood-brain barrier through activating AMP-activated protein kinase in old mice.
Wang X, Xue GX, Liu WC, Shu H, Wang M, Sun Y, Liu X, Sun YE, Liu CF, Liu J, Liu W, Jin X
Aging cell 2017 Apr;16(2):414-421
Aging cell 2017 Apr;16(2):414-421
Potent Vasoconstrictor Kisspeptin-10 Induces Atherosclerotic Plaque Progression and Instability: Reversal by its Receptor GPR54 Antagonist.
Sato K, Shirai R, Hontani M, Shinooka R, Hasegawa A, Kichise T, Yamashita T, Yoshizawa H, Watanabe R, Matsuyama TA, Ishibashi-Ueda H, Koba S, Kobayashi Y, Hirano T, Watanabe T
Journal of the American Heart Association 2017 Apr 14;6(4)
Journal of the American Heart Association 2017 Apr 14;6(4)
Impaired Epidermal to Dendritic T Cell Signaling Slows Wound Repair in Aged Skin.
Keyes BE, Liu S, Asare A, Naik S, Levorse J, Polak L, Lu CP, Nikolova M, Pasolli HA, Fuchs E
Cell 2016 Nov 17;167(5):1323-1338.e14
Cell 2016 Nov 17;167(5):1323-1338.e14
Chimeric Mice with Competent Hematopoietic Immunity Reproduce Key Features of Severe Lassa Fever.
Oestereich L, Lüdtke A, Ruibal P, Pallasch E, Kerber R, Rieger T, Wurr S, Bockholt S, Pérez-Girón JV, Krasemann S, Günther S, Muñoz-Fontela C
PLoS pathogens 2016 May;12(5):e1005656
PLoS pathogens 2016 May;12(5):e1005656
Antitumor cytotoxicity induced by bone-marrow-derived antigen-presenting cells is facilitated by the tumor suppressor protein p53 via regulation of IL-12.
Slatter TL, Wilson M, Tang C, Campbell HG, Ward VK, Young VL, Van Ly D, Fleming NI, Braithwaite AW, Baird MA
Oncoimmunology 2016 Mar;5(3):e1112941
Oncoimmunology 2016 Mar;5(3):e1112941
Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia.
Himmelein S, Lindemann A, Sinicina I, Strupp M, Brandt T, Hüfner K
Journal of virology 2015 May;89(10):5747-50
Journal of virology 2015 May;89(10):5747-50
The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
Prevete N, Liotti F, Visciano C, Marone G, Melillo RM, de Paulis A
Oncogene 2015 Jul;34(29):3826-38
Oncogene 2015 Jul;34(29):3826-38
Edaravone protect against retinal damage in streptozotocin-induced diabetic mice.
Yuan D, Xu Y, Hang H, Liu X, Chen X, Xie P, Yuan S, Zhang W, Lin X, Liu Q
PloS one 2014;9(6):e99219
PloS one 2014;9(6):e99219
Evaluation of antiviral efficacy of ribavirin, arbidol, and T-705 (favipiravir) in a mouse model for Crimean-Congo hemorrhagic fever.
Oestereich L, Rieger T, Neumann M, Bernreuther C, Lehmann M, Krasemann S, Wurr S, Emmerich P, de Lamballerie X, Ölschläger S, Günther S
PLoS neglected tropical diseases 2014 May;8(5):e2804
PLoS neglected tropical diseases 2014 May;8(5):e2804
Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-β.
Wiener Z, Band AM, Kallio P, Högström J, Hyvönen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):E2229-36
Proceedings of the National Academy of Sciences of the United States of America 2014 May 27;111(21):E2229-36
Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-κB downregulation.
Liu L, Salnikov AV, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Schemmer P, Werner J, Herr I
International journal of cancer 2014 May 15;134(10):2489-503
International journal of cancer 2014 May 15;134(10):2489-503
Proinflammatory cytokines differentially regulate adipocyte mitochondrial metabolism, oxidative stress, and dynamics.
Hahn WS, Kuzmicic J, Burrill JS, Donoghue MA, Foncea R, Jensen MD, Lavandero S, Arriaga EA, Bernlohr DA
American journal of physiology. Endocrinology and metabolism 2014 May 1;306(9):E1033-45
American journal of physiology. Endocrinology and metabolism 2014 May 1;306(9):E1033-45
The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.
Diefenbacher ME, Popov N, Blake SM, Schülein-Völk C, Nye E, Spencer-Dene B, Jaenicke LA, Eilers M, Behrens A
The Journal of clinical investigation 2014 Aug;124(8):3407-18
The Journal of clinical investigation 2014 Aug;124(8):3407-18
Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
Janek T, Krasowska A, Radwańska A, Łukaszewicz M
PloS one 2013;8(3):e57991
PloS one 2013;8(3):e57991
The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
Hage C, Rausch V, Giese N, Giese T, Schönsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
Cell death & disease 2013 May 9;4:e627
Cell death & disease 2013 May 9;4:e627
The ROS scavenger, NAC, regulates hepatic Vα14iNKT cells signaling during Fas mAb-dependent fulminant liver failure.
Downs I, Liu J, Aw TY, Adegboyega PA, Ajuebor MN
PloS one 2012;7(6):e38051
PloS one 2012;7(6):e38051
FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis.
Goldie SJ, Mulder KW, Tan DW, Lyons SK, Sims AH, Watt FM
Cancer research 2012 Jul 1;72(13):3424-36
Cancer research 2012 Jul 1;72(13):3424-36
Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
Journal of molecular and cellular cardiology 2010 Jun;48(6):1225-35
Journal of molecular and cellular cardiology 2010 Jun;48(6):1225-35
In-vivo transfection of pcDNA3.1-IGFBP7 inhibits melanoma growth in mice through apoptosis induction and VEGF downexpression.
Chen RY, Chen HX, Lin JX, She WB, Jiang P, Xu L, Tu YT
Journal of experimental & clinical cancer research : CR 2010 Feb 16;29(1):13
Journal of experimental & clinical cancer research : CR 2010 Feb 16;29(1):13
Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
Apoptosis : an international journal on programmed cell death 2010 Apr;15(4):463-73
Apoptosis : an international journal on programmed cell death 2010 Apr;15(4):463-73
Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Facchini A, Flamigni F, Santi S, Facchini A, Borzi' RM
Journal of cellular physiology 2007 Feb;210(2):417-27
Journal of cellular physiology 2007 Feb;210(2):417-27
Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury.
Terayama R, Bando Y, Murakami K, Kato K, Kishibe M, Yoshida S
Neuroscience 2007 Aug 10;148(1):175-87
Neuroscience 2007 Aug 10;148(1):175-87
Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells.
Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaän-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW
Journal of cell science 2006 Oct 15;119(Pt 20):4247-56
Journal of cell science 2006 Oct 15;119(Pt 20):4247-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Cleaved Caspase-3 (Asp175) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cleaved Caspase-3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Caspase-3 in Jurkat Human Cell Line. Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin using Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Caspase-3 in Human Colon Cancer Tissue. Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Cleaved Caspase-3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 μM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase-3 Monoclonal Antibody (Catalog # MAB835, filled histogram) followed by anti-Rabbit IgG FITC-conjugated secondary antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.
