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LearnAP2922c
antibody from Abgent
Targeting: PDIA3
ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP2922c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP2922c, RRID:AB_1967768
- Product name
- PDIA3 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Western blot analysis of PDIA3 Antibody (Center) (Cat. #AP2922c) in A2058, A375, Ramos cell line lysates (35ug/lane). PDIA3 (arrow) was detected using the purified Pab.(2ug/ml)"
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of PDIA3 (arrow) using rabbit polyclonal PDIA3 Antibody (Center) (Cat. #AP2922c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDIA3 gene (Lane 2) .
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PDIA3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Flow cytometric analysis of widr cells using PDIA3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
