Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB10725 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10725, RRID:AB_11188085
- Product name
- KRT19 monoclonal antibody, clone 4E8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant KRT19.
- Isotype
- IgG
- Antibody clone number
- 4E8
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using KRT19 monoclonal antibody, clone 4E8 (Cat # MAB10725) against human KRT19 (aa 115-269) recombinant protein. (Expected MW is 43.1 kDa)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using KRT19 monoclonal antibody, clone 4E8 (Cat # MAB10725) against T-47D (1) , MCF-7 (2) , HepG2 (3) and SW-620 (4) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of KRT19 monoclonal antibody, clone 4E8 (Cat # MAB10725) .
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using KRT19 monoclonal antibody, clone 4E8 (Cat # MAB10725) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human cervical cancer (A) , human colon cancer (B) , human stomach cancer (C) and human bladder cancer (D) tissues using KRT19 monoclonal antibody, clone 4E8 (Cat # MAB10725) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)