Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM08361PU-M - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Cytokeratin 19
- Antibody type
- Monoclonal
- Antigen
- Human mammary carcinoma cell line MCF-7
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- A53-B/A2
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
Submitted references Primary culture of breast cancer: a model system for epithelial-mesenchymal transition and cancer stem cells.
Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.
Nishikata T, Ishikawa M, Matsuyama T, Takamatsu K, Fukuhara T, Konishi Y
Anticancer research 2013 Jul;33(7):2867-73
Anticancer research 2013 Jul;33(7):2867-73
Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.
Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H
European journal of cancer & clinical oncology 1985 Jun;21(6):733-40
European journal of cancer & clinical oncology 1985 Jun;21(6):733-40
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Cytokeratin 19 Antibody Cat.-No AM08361PU-M (Clone A53-B/A2). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- MCF-7 cells were stained with monoclonal anti-Cytokeratin 19 Antibody Cat.-No AM08361PU-M (Clone A53-B/A2), followed by Alexa Fluor® 546 secondary antibody and DAPI (nuclei). Images were aquired on a Nikon FC300 inverted microscope at 20X magnification. Data provided by Dr. John Nolan, La Jolla Bioengineering Institute.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Embedded Human Kidney tissue stained with monoclonal anti-Cytokeratin 19 Antibody Cat.-No AM08361PU-M (Clone A53-B/A2) at 10 µg/ml after heat-induced antigen retrieval.
