Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 36-3003 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-CK19 Antibody
- Antibody type
- Monoclonal
- Description
- Keratin-19 (K19) is a type I intermediate filament protein and one of the best characterized of the keratins expressed in mature striated muscle. Keratin-19 is expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium. Keratin-19 has been used as a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Immunohistochemical data has shown that Keratin-19 may be uses as a marker for human skin stem cells.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Human breast cancer MCF-7 cells wer
e used as the immunogen for the Ker
atin-19 antibody.- Isotype
- IgG
- Antibody clone number
- A53-B/A2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CK19. Anti CK19 antibody (Clone: A53-B/A2) was used at 1 µg/ml in 1) HepG2 and 2) MCF7 lysates.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of CK19. Anti CK19 antibody (Clone: A53-B/A2) was used at 1 µg/ml in (1) MIapica-2, 0.1 µg/ml in (2) PANC-1, 0.01 µg/ml in (3) HCT-116 and (4) PC3 lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CK19 in human colon cancer using CK19 antibody (Clone: A53-B/A2) at 1:100 dilution.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CK19 in human Pancrease, Duct using CK19 antibody (Clone: A53-B/A2) at 10 µg/ml.
- Protocol
- Protocol