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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-12613 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Cytokeratin 19 Monoclonal Antibody (RCK108)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-12613 targets Cytokeratin 19 in IHC (P) and WB applications and shows reactivity with Human samples. The MA5-12613 immunogen is cytoskeleton preparation of T24 cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- RCK108
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C
Submitted references Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON.
WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis.
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Kuga T, Kume H, Adachi J, Kawasaki N, Shimizu M, Hoshino I, Matsubara H, Saito Y, Nakayama Y, Tomonaga T
Scientific reports 2016 Sep 29;6:34472
Scientific reports 2016 Sep 29;6:34472
WNT7A and PAX6 define corneal epithelium homeostasis and pathogenesis.
Ouyang H, Xue Y, Lin Y, Zhang X, Xi L, Patel S, Cai H, Luo J, Zhang M, Zhang M, Yang Y, Li G, Li H, Jiang W, Yeh E, Lin J, Pei M, Zhu J, Cao G, Zhang L, Yu B, Chen S, Fu XD, Liu Y, Zhang K
Nature 2014 Jul 17;511(7509):358-61
Nature 2014 Jul 17;511(7509):358-61
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39
Diagnostic pathology 2012 Apr 12;7:39
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Cytokeratin 19 using Cytokeratin 19 Monoclonal Antibody (Product # MA5-12613) on LS174T Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Whole cell extracts of MCF7 (Lane 1), T-47D (Lane 2), HT-29 (Lane 3), Caco-2 (Lane 4), HCT 116 (Lane 5) and COLO 205 (Lane 6). The blot was probed with Anti- Cytokeratin 19 Mouse Monoclonal Antibody (Product # MA5-12613, 1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 44 kDa band corresponding to Cytokeratin 19 was observed across the cell lines tested and an additional band at 38 kDa could be the result of cross reactivity with other cytokeratins. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human colon carcinoma stained with Keratin 19 antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Cytokeratin 19 was done on MCF7 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Cytokeratin 19 Mouse Monoclonal Antibody (MA5-12613, red histogram) or with mouse isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10, 000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytokeratin 19 was performed using 90% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 (RCK108) Mouse Monoclonal Antibody (Product # MA5-12613) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
