Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AF3506 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cytokeratin 19 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Cytokeratin 19 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human Cytokeratin 14 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P08727
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cytokeratin 19 by Western Blot. Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, DU145 human prostate carcinoma cell line, HCT-116 human colorectal carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cytokeratin 19 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cytokeratin 19 by Simple WesternTM. Simple Western lane view shows lysates of HT-29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cytokeratin 19 at approximately 50 kDa (as indicated) using 1 µg/mL of Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytokeratin 19 in MCF-7 Human Cell Line. Cytokeratin 19 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytokeratin 19 in Human Kidney. Cytokeratin 19 was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Cytokeratin 19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3506) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of KRT19 in Human MCF-7 cell line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Sheep Anti-Human KRT19 Affinity Purified Polyclonal Antibody (Catalog # AF3506, filled histogram) or control antibody (Catalog # 5-001-A, open histogram) followed by Anti-Sheep IgG PE-conjugated secondary antibody (Catalog # F0126). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.