PA1-46159

Soni H, Kumar R, Kanthakumar P, Adebiyi A
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Jul;35(7):e21729

Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells.

Cojocaru F, Şelescu T, Domocoş D, Măruţescu L, Chiritoiu G, Chelaru NR, Dima S, Mihăilescu D, Babes A, Cucu D
Scientific reports 2021 Jan 21;11(1):2018

Thermo-TRPs and gut microbiota are involved in thermogenesis and energy metabolism during low temperature exposure of obese mice.

Wen J, Bo T, Zhang X, Wang Z, Wang D
The Journal of experimental biology 2020 Jun 4;223(Pt 11)

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of TRPA1 in human brain membrane fraction using 2 µg/mL (Product # PA1-46159).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry analysis of TRPA1 in HEK 293 cells (Flp In Trex system) cells. Samples were incubated in TRPA1 polyclonal antibody (Product # PA1-46159).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of TRPA1 in mouse intestine. Samples were incubated in TRPA1 polyclonal antibody (Product # PA1-46159).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of TRPA1 in A549 cells. Samples were incubated in TRPA1 polyclonal antibody (Product # PA1-46159) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 650.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Expression of TRPA1 in human PDAC cell lines. ( A ) Expression pattern of the TRPA1 protein in PDAC cell lines. Blots from whole-cell lysates (50 µg) obtained from three PDAC cell lines, the human pancreatic ductal cells, wild-type HEK293T and HEK293T/A1-transfected cell lines were probed with antibodies against TRPA1 and Hsp90 on the same gel. Cropped gel is presented here with full gel available in Supplemental Fig. 1 . ( B ) Densitometry quantification of the bands from panel A was performed with Quantity One software. The results are expressed as the ratio of the expression of TRPA1 to Hsp 90. ( C ) Levels of TRPA1 mRNA were determined by real-time RT-PCR, and a comparative Ct method (2 -DeltaDeltaCt ) was used for the relative mRNA quantification. Hu18S was used as endogenous gene. The values in ( B , C ) are shown as the means +- SD; n = 3. * p < 0.05 vs. control.