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- Antibody Data
- Antigen structure
- References [32]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB110-40763 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40763, RRID:AB_715124
- Product name
- Rabbit Polyclonal TRPA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Transient receptor potential ankyrin1 channel is endogenously expressed in T cells and is involved in immune functions.
Knockout of TRPA1 exacerbates angiotensin II-induced kidney injury.
Engagement of MicroRNA-155 in Exaggerated Oxidative Stress Signal and TRPA1 in the Dorsal Horn of the Spinal Cord and Neuropathic Pain During Chemotherapeutic Oxaliplatin.
CGRPergic Nerve TRPA1 Channels Contribute to Epigallocatechin Gallate-Induced Neurogenic Vasodilation.
Catechol estrogens stimulate insulin secretion in pancreatic β-cells via activation of the transient receptor potential A1 (TRPA1) channel.
Validation of antibodies for the specific detection of human TRPA1.
DF2726A, a new IL-8 signalling inhibitor, is able to counteract chemotherapy-induced neuropathic pain.
Expression and Activity of TRPA1 and TRPV1 in the Intervertebral Disc: Association with Inflammation and Matrix Remodeling.
TRPA1 channel contributes to myocardial ischemia-reperfusion injury.
TRPA1 inhibition ameliorates pressure overload-induced cardiac hypertrophy and fibrosis in mice.
Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance.
Selective killing of proinflammatory synovial fibroblasts via activation of transient receptor potential ankyrin (TRPA1).
TRPA1 expression is downregulated by dexamethasone and aurothiomalate in human chondrocytes: TRPA1 as a novel factor and drug target in arthritis.
Mast cell hyperactivity underpins the development of oxygen-induced retinopathy.
Loss of Transient Receptor Potential Ankyrin 1 Channel Deregulates Emotion, Learning and Memory, Cognition, and Social Behavior in Mice.
The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin.
Notch1-promoted TRPA1 expression in erythroleukemic cells suppresses erythroid but enhances megakaryocyte differentiation.
Schwann cell TRPA1 mediates neuroinflammation that sustains macrophage-dependent neuropathic pain in mice.
Transient Receptor Potential Ankyrin 1 Activation within the Cardiac Myocyte Limits Ischemia-reperfusion Injury in Rodents.
Transient receptor potential ankyrin 1 (TRPA1) is functionally expressed in primary human osteoarthritic chondrocytes.
Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease.
TRPA1 mediates the effects of hypothermia on the monocyte inflammatory response.
Roles of Cav3.2 and TRPA1 channels targeted by hydrogen sulfide in pancreatic nociceptive processing in mice with or without acute pancreatitis.
Tmem100 Is a Regulator of TRPA1-TRPV1 Complex and Contributes to Persistent Pain.
A Potent and Site-Selective Agonist of TRPA1.
Transient receptor potential vanilloid 4 (TRPV4) is downregulated in keratinocytes in human non-melanoma skin cancer.
TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis.
Hydrogen sulfide-induced mechanical hyperalgesia and allodynia require activation of both Cav3.2 and TRPA1 channels in mice.
Expression of functional TRPA1 receptor on human lung fibroblast and epithelial cells.
Transient receptor potential ankyrin 1 is expressed by inhibitory motoneurons of the mouse intestine.
Transient receptor potential ankyrin-1 has a major role in mediating visceral pain in mice.
TRPA1 in mast cell activation-induced long-lasting mechanical hypersensitivity of vagal afferent C-fibers in guinea pig esophagus.
Sahoo SS, Majhi RK, Tiwari A, Acharya T, Kumar PS, Saha S, Kumar A, Goswami C, Chattopadhyay S
Bioscience reports 2019 Sep 30;39(9)
Bioscience reports 2019 Sep 30;39(9)
Knockout of TRPA1 exacerbates angiotensin II-induced kidney injury.
Ma S, Zhang Y, He K, Wang P, Wang DH
American journal of physiology. Renal physiology 2019 Sep 1;317(3):F623-F631
American journal of physiology. Renal physiology 2019 Sep 1;317(3):F623-F631
Engagement of MicroRNA-155 in Exaggerated Oxidative Stress Signal and TRPA1 in the Dorsal Horn of the Spinal Cord and Neuropathic Pain During Chemotherapeutic Oxaliplatin.
Miao F, Wang R, Cui G, Li X, Wang T, Li X
Neurotoxicity research 2019 Nov;36(4):712-723
Neurotoxicity research 2019 Nov;36(4):712-723
CGRPergic Nerve TRPA1 Channels Contribute to Epigallocatechin Gallate-Induced Neurogenic Vasodilation.
Peixoto-Neves D, Soni H, Adebiyi A
ACS chemical neuroscience 2019 Jan 16;10(1):216-220
ACS chemical neuroscience 2019 Jan 16;10(1):216-220
Catechol estrogens stimulate insulin secretion in pancreatic β-cells via activation of the transient receptor potential A1 (TRPA1) channel.
Ma W, Chen X, Cerne R, Syed SK, Ficorilli JV, Cabrera O, Obukhov AG, Efanov AM
The Journal of biological chemistry 2019 Feb 22;294(8):2935-2946
The Journal of biological chemistry 2019 Feb 22;294(8):2935-2946
Validation of antibodies for the specific detection of human TRPA1.
Virk HS, Rekas MZ, Biddle MS, Wright AKA, Sousa J, Weston CA, Chachi L, Roach KM, Bradding P
Scientific reports 2019 Dec 6;9(1):18500
Scientific reports 2019 Dec 6;9(1):18500
DF2726A, a new IL-8 signalling inhibitor, is able to counteract chemotherapy-induced neuropathic pain.
Brandolini L, Castelli V, Aramini A, Giorgio C, Bianchini G, Russo R, De Caro C, d'Angelo M, Catanesi M, Benedetti E, Giordano A, Cimini A, Allegretti M
Scientific reports 2019 Aug 13;9(1):11729
Scientific reports 2019 Aug 13;9(1):11729
Expression and Activity of TRPA1 and TRPV1 in the Intervertebral Disc: Association with Inflammation and Matrix Remodeling.
Kameda T, Zvick J, Vuk M, Sadowska A, Tam WK, Leung VY, Bölcskei K, Helyes Z, Applegate LA, Hausmann ON, Klasen J, Krupkova O, Wuertz-Kozak K
International journal of molecular sciences 2019 Apr 10;20(7)
International journal of molecular sciences 2019 Apr 10;20(7)
TRPA1 channel contributes to myocardial ischemia-reperfusion injury.
Conklin DJ, Guo Y, Nystoriak MA, Jagatheesan G, Obal D, Kilfoil PJ, Hoetker JD, Guo L, Bolli R, Bhatnagar A
American journal of physiology. Heart and circulatory physiology 2019 Apr 1;316(4):H889-H899
American journal of physiology. Heart and circulatory physiology 2019 Apr 1;316(4):H889-H899
TRPA1 inhibition ameliorates pressure overload-induced cardiac hypertrophy and fibrosis in mice.
Wang Z, Xu Y, Wang M, Ye J, Liu J, Jiang H, Ye D, Wan J
EBioMedicine 2018 Oct;36:54-62
EBioMedicine 2018 Oct;36:54-62
Cancer Cells Co-opt the Neuronal Redox-Sensing Channel TRPA1 to Promote Oxidative-Stress Tolerance.
Takahashi N, Chen HY, Harris IS, Stover DG, Selfors LM, Bronson RT, Deraedt T, Cichowski K, Welm AL, Mori Y, Mills GB, Brugge JS
Cancer cell 2018 Jun 11;33(6):985-1003.e7
Cancer cell 2018 Jun 11;33(6):985-1003.e7
Selective killing of proinflammatory synovial fibroblasts via activation of transient receptor potential ankyrin (TRPA1).
Lowin T, Bleck J, Schneider M, Pongratz G
Biochemical pharmacology 2018 Aug;154:293-302
Biochemical pharmacology 2018 Aug;154:293-302
TRPA1 expression is downregulated by dexamethasone and aurothiomalate in human chondrocytes: TRPA1 as a novel factor and drug target in arthritis.
Nummenmaa E, Hämäläinen M, Moilanen LJ, Moilanen T, Vuolteenaho K, Moilanen E
RMD open 2017;3(2):e000556
RMD open 2017;3(2):e000556
Mast cell hyperactivity underpins the development of oxygen-induced retinopathy.
Matsuda K, Okamoto N, Kondo M, Arkwright PD, Karasawa K, Ishizaka S, Yokota S, Matsuda A, Jung K, Oida K, Amagai Y, Jang H, Noda E, Kakinuma R, Yasui K, Kaku U, Mori Y, Onai N, Ohteki T, Tanaka A, Matsuda H
The Journal of clinical investigation 2017 Nov 1;127(11):3987-4000
The Journal of clinical investigation 2017 Nov 1;127(11):3987-4000
Loss of Transient Receptor Potential Ankyrin 1 Channel Deregulates Emotion, Learning and Memory, Cognition, and Social Behavior in Mice.
Lee KI, Lin HC, Lee HT, Tsai FC, Lee TS
Molecular neurobiology 2017 Jul;54(5):3606-3617
Molecular neurobiology 2017 Jul;54(5):3606-3617
The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin.
Hidaka T, Ogawa E, Kobayashi EH, Suzuki T, Funayama R, Nagashima T, Fujimura T, Aiba S, Nakayama K, Okuyama R, Yamamoto M
Nature immunology 2017 Jan;18(1):64-73
Nature immunology 2017 Jan;18(1):64-73
Notch1-promoted TRPA1 expression in erythroleukemic cells suppresses erythroid but enhances megakaryocyte differentiation.
Chen JL, Ping YH, Tseng MJ, Chang YI, Lee HC, Hsieh RH, Yeh TS
Scientific reports 2017 Feb 21;7:42883
Scientific reports 2017 Feb 21;7:42883
Schwann cell TRPA1 mediates neuroinflammation that sustains macrophage-dependent neuropathic pain in mice.
De Logu F, Nassini R, Materazzi S, Carvalho Gonçalves M, Nosi D, Rossi Degl'Innocenti D, Marone IM, Ferreira J, Li Puma S, Benemei S, Trevisan G, Souza Monteiro de Araújo D, Patacchini R, Bunnett NW, Geppetti P
Nature communications 2017 Dec 1;8(1):1887
Nature communications 2017 Dec 1;8(1):1887
Transient Receptor Potential Ankyrin 1 Activation within the Cardiac Myocyte Limits Ischemia-reperfusion Injury in Rodents.
Lu Y, Piplani H, McAllister SL, Hurt CM, Gross ER
Anesthesiology 2016 Dec;125(6):1171-1180
Anesthesiology 2016 Dec;125(6):1171-1180
Transient receptor potential ankyrin 1 (TRPA1) is functionally expressed in primary human osteoarthritic chondrocytes.
Nummenmaa E, Hämäläinen M, Moilanen LJ, Paukkeri EL, Nieminen RM, Moilanen T, Vuolteenaho K, Moilanen E
Arthritis research & therapy 2016 Aug 11;18(1):185
Arthritis research & therapy 2016 Aug 11;18(1):185
Role of transient receptor potential ankyrin 1 channels in Alzheimer's disease.
Lee KI, Lee HT, Lin HC, Tsay HJ, Tsai FC, Shyue SK, Lee TS
Journal of neuroinflammation 2016 Apr 27;13(1):92
Journal of neuroinflammation 2016 Apr 27;13(1):92
TRPA1 mediates the effects of hypothermia on the monocyte inflammatory response.
Billeter AT, Galbraith N, Walker S, Lawson C, Gardner SA, Sarojini H, Galandiuk S, Polk HC Jr
Surgery 2015 Sep;158(3):646-54
Surgery 2015 Sep;158(3):646-54
Roles of Cav3.2 and TRPA1 channels targeted by hydrogen sulfide in pancreatic nociceptive processing in mice with or without acute pancreatitis.
Terada Y, Fujimura M, Nishimura S, Tsubota M, Sekiguchi F, Kawabata A
Journal of neuroscience research 2015 Feb;93(2):361-9
Journal of neuroscience research 2015 Feb;93(2):361-9
Tmem100 Is a Regulator of TRPA1-TRPV1 Complex and Contributes to Persistent Pain.
Weng HJ, Patel KN, Jeske NA, Bierbower SM, Zou W, Tiwari V, Zheng Q, Tang Z, Mo GC, Wang Y, Geng Y, Zhang J, Guan Y, Akopian AN, Dong X
Neuron 2015 Feb 18;85(4):833-46
Neuron 2015 Feb 18;85(4):833-46
A Potent and Site-Selective Agonist of TRPA1.
Takaya J, Mio K, Shiraishi T, Kurokawa T, Otsuka S, Mori Y, Uesugi M
Journal of the American Chemical Society 2015 Dec 23;137(50):15859-64
Journal of the American Chemical Society 2015 Dec 23;137(50):15859-64
Transient receptor potential vanilloid 4 (TRPV4) is downregulated in keratinocytes in human non-melanoma skin cancer.
Fusi C, Materazzi S, Minocci D, Maio V, Oranges T, Massi D, Nassini R
The Journal of investigative dermatology 2014 Sep;134(9):2408-2417
The Journal of investigative dermatology 2014 Sep;134(9):2408-2417
TRPA1-dependent pruritus in IL-13-induced chronic atopic dermatitis.
Oh MH, Oh SY, Lu J, Lou H, Myers AC, Zhu Z, Zheng T
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5371-82
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5371-82
Hydrogen sulfide-induced mechanical hyperalgesia and allodynia require activation of both Cav3.2 and TRPA1 channels in mice.
Okubo K, Matsumura M, Kawaishi Y, Aoki Y, Matsunami M, Okawa Y, Sekiguchi F, Kawabata A
British journal of pharmacology 2012 Jul;166(5):1738-43
British journal of pharmacology 2012 Jul;166(5):1738-43
Expression of functional TRPA1 receptor on human lung fibroblast and epithelial cells.
Mukhopadhyay I, Gomes P, Aranake S, Shetty M, Karnik P, Damle M, Kuruganti S, Thorat S, Khairatkar-Joshi N
Journal of receptor and signal transduction research 2011 Oct;31(5):350-8
Journal of receptor and signal transduction research 2011 Oct;31(5):350-8
Transient receptor potential ankyrin 1 is expressed by inhibitory motoneurons of the mouse intestine.
Poole DP, Pelayo JC, Cattaruzza F, Kuo YM, Gai G, Chiu JV, Bron R, Furness JB, Grady EF, Bunnett NW
Gastroenterology 2011 Aug;141(2):565-75, 575.e1-4
Gastroenterology 2011 Aug;141(2):565-75, 575.e1-4
Transient receptor potential ankyrin-1 has a major role in mediating visceral pain in mice.
Cattaruzza F, Spreadbury I, Miranda-Morales M, Grady EF, Vanner S, Bunnett NW
American journal of physiology. Gastrointestinal and liver physiology 2010 Jan;298(1):G81-91
American journal of physiology. Gastrointestinal and liver physiology 2010 Jan;298(1):G81-91
TRPA1 in mast cell activation-induced long-lasting mechanical hypersensitivity of vagal afferent C-fibers in guinea pig esophagus.
Yu S, Gao G, Peterson BZ, Ouyang A
American journal of physiology. Gastrointestinal and liver physiology 2009 Jul;297(1):G34-42
American journal of physiology. Gastrointestinal and liver physiology 2009 Jul;297(1):G34-42
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRPA1 Antibody [NB110-40763] - Detection of TRPA1 in human brain membrane fraction using 2ug/ml of NB110-40763.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TRPA1 Antibody [NB110-40763] - Staining TRPA1 in mouse intestine.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TRPA1 Antibody [NB110-40763] - An intracellular stain was performed on A549 cells with NB110-40763C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
