Explore
Validate
Learn
Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 10105-2-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2T/HSPC150 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MQRASRLKR ELHMLATEPP PGITCWQDKD QMDDLRAQIL GGANTPYEKG VFKLEVIIPE RYPFEPPQIR FLTPIYHPNI DSAGRICLDV LKLPPKGAWR PSLNIATVLT SIQLLMSEPN PDDPLMADIS SEFKYNKPAF LKNARQWTEK HARQKQKADE EEMLDNLPEA GDSRVHNSTQ KRKASQLVGI EKKFHPDV (1-197 aa encoded by BC004152)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-UBE2T/HSPC150 (IP:10105-2-AP, 3ug; Detection:10105-2-AP 1:500) with HeLa cells lysate 3000ug.
