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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG54028 - Provider product page
- Provider
- Arigo
- Product name
- anti-CHD3 antibody (C-term)
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4B1-F1-G4
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: CHD3 (C-terminus) in Y79 and Raji cell lysates stained with CHD3 antibody [4B1-F1-G4]inal (ARG54028) (diluted in 1: 1000). Predicted band size: 226KDa. Observed band size: 260KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human colon stained with CHD3 antibody [4B1-F1-G4], C-terminal (ARG54028) (diluted in 1/500-1/1000).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: K562 cells stained with CHD3 antibody [4B1-F1-G4], C-terminal (ARG54028) (red, 1/100), followed by FITC-conjugated goat mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
