Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-88312 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88312, RRID:AB_11018687
- Product name
- Rabbit Polyclonal TPX2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human TPX2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TPX2 Antibody [NBP1-88312] - Analysis in human cell line RH-30.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TPX2 Antibody [NBP1-88312] - Analysis in control (vector only transfected HEK293T lysate) and TPX2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TPX2 Antibody [NBP1-88312] - Staining in human testis and pancreas tissues using anti-TPX2 antibody. Corresponding TPX2 RNA-seq data are presented for the same tissues.