PAB28628

antibody from Abnova Corporation

Targeting: NCAPH BRRN1, CAP-H, hCAP-H

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28628

Provider

Abnova Corporation

Product name

NCAPH polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NCAPH.

Antigen sequence

KFTNTQITEHYSTCIKLSTENKITTKNAFGLHLID
FMSEILKQKDTEPTNFKVAAGTLDASTKIYAVRVD
AVHADVYRVLGGLGKDAPSLEEVEGHVADGSATEM
GTTKKAVKPKKKHLHRTIEQNINNLNVSEADRKCE
I


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp with NCAPH polyclonal antibody (Cat # PAB28628) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with NCAPH polyclonal antibody (Cat # PAB28628) at 1-4 ug/ml shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human heart with NCAPH polyclonal antibody (Cat # PAB28628) shows strong cytoplasmic positivity in myocytes at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)