PAB28677

antibody from Abnova Corporation

Targeting: NCAPH BRRN1, CAP-H, hCAP-H

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28677

Provider

Abnova Corporation

Product name

NCAPH polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant NCAPH, subunit H.

Antigen sequence

LHCQDYRSELLFPSDVQTLSTGEPLELPELGCVEM
TDLKAPLQQCAEDRQICPSLAGFQFTQWDSETHNE
SVSALVDKFKKNDQVFDINAEVDESDCGDFPDGSL
GDDFDANDEPDHT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot anyalysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with NCAPH polyclonal antibody (Cat # PAB28677).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with NCAPH polyclonal antibody (Cat # PAB28677) at 1-4 ug/mL shows positivity in cytoplasm & cytoskeleton (microtubules).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil with NCAPH polyclonal antibody (Cat # PAB28677) shows cytoplasmic positivity in reaction center cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry