LS-C815384

antibody from LSBio

Targeting: NCAPH BRRN1, CAP-H, hCAP-H

Western blot (Data presented on provider website)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C815384

Provider

LSBio

Product name

NCAPH / CAP-H Antibody (Preservative Free) LS-C815384

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

NCAPH was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-NCAPH rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-NCAPH rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 83 kDa. Observed band size: 110 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

NCAPH was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-NCAPH rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-NCAPH rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 83 kDa. Observed band size: 110 kDa.