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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-30467 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NCAPH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa.
- Concentration
- 0.68 mg/mL
Submitted references Expression and Clinical Significance of the NCAPH, AGGF1, and FOXC2 Proteins in Serous Ovarian Cancer.
Sun Y, Wang X, Wen H, Zhu B, Yu L
Cancer management and research 2021;13:7253-7262
Cancer management and research 2021;13:7253-7262
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NCAPH Polyclonal Antibody (Product # PA5-30467). Sample (30 µg of whole cell lysate). Lane A: HeLa . 7.5% SDS PAGE. NCAPH Polyclonal Antibody (Product # PA5-30467) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NCAPH Polyclonal Antibody [N1N2], N-term detects BRRN1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: BRRN1 protein stained by NCAPH Polyclonal Antibody [N1N2], N-term (Product # PA5-30467) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BRRN1 (Product # PA5-30467) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
