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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- OAAB02693 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- SRD5A3 antibody Affinity Purified Rabbit Polyclonal antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- SRD5A3 Antibody (C-term) western blot analysis in NCI-H292,293 cell lysates (35ug/lane). ÊThis demonstrates the SRD5A3 antibody detected the SRD5A3 protein (arrow).
- Sample type
- NCI-H292,293 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- SRD5A3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SRD5A3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- human prostate carcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- SRD5A3 Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- K562 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
