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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [2]
- Other assay [1]
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- Product number
- 17-9475-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ID2 Monoclonal Antibody (ILCID2), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- Description: This ILCID2 monoclonal antibody recognizes mouse and human ID2. This ILCID2 antibody requires Foxp3/Transcription Factor Staining Buffer set and will perform poorly when used with standard IC Fixation & Permeabilization Buffer Set and protocol. Applications Reported: This ILCID2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This ILCID2 antibody has been tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- ILCID2
- Vial size
- 25 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Insulin-like Growth Factor 1 Supports a Pulmonary Niche that Promotes Type 3 Innate Lymphoid Cell Development in Newborn Lungs.
Transcriptome dynamics of CD4(+) T cells during malaria maps gradual transit from effector to memory.
Oherle K, Acker E, Bonfield M, Wang T, Gray J, Lang I, Bridges J, Lewkowich I, Xu Y, Ahlfeld S, Zacharias W, Alenghat T, Deshmukh H
Immunity 2020 Feb 18;52(2):275-294.e9
Immunity 2020 Feb 18;52(2):275-294.e9
Transcriptome dynamics of CD4(+) T cells during malaria maps gradual transit from effector to memory.
Soon MSF, Lee HJ, Engel JA, Straube J, Thomas BS, Pernold CPS, Clarke LS, Laohamonthonkul P, Haldar RN, Williams CG, Lansink LIM, Moreira ML, Bramhall M, Koufariotis LT, Wood S, Chen X, James KR, Lönnberg T, Lane SW, Belz GT, Engwerda CR, Khoury DS, Davenport MP, Svensson V, Teichmann SA, Haque A
Nature immunology 2020 Dec;21(12):1597-1610
Nature immunology 2020 Dec;21(12):1597-1610
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C57BL/6 mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 1.0 µg of Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (blue histogram) or 1.0 µg of ID2 Monoclonal Antibody, APC (purple histogram). Splenocytes within the lymphocyte gate positive for NK1.1 Monoclonal Antibody, eFluor 450 (Product # 48-5941-82) and CD49b Monoclonal Antibody, eFluor 450 (Product # 48-5971-82) were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C57BL/6 mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with either 1.0 µg of Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or 1.0 µg of ID2 Monoclonal Antibody, APC (right). Cells were co-stained and gated based on the expression of both NK1.1 Monoclonal Antibody, eFluor 450 (Product # 48-5941-82) and CD49b Monoclonal Antibody, eFluor 450 (Product # 48-5971-82) (purple histogram); CD8a Monoclonal Antibody, PE-Cyanine 7 (Product # 25-0081-82) (blue histogram); CD4 Monoclonal Antibody, FITC (Product # 11-0042-82) (orange histogram); CD3 Monoclonal Antibody (Product # 11-0032-82) (green histogram). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
