STJ11103757

antibody from St John's Laboratory

Targeting: FBH1 Fbx18, FBXO18, FLJ14590

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

STJ11103757

Provider

St John's Laboratory

Product name

Anti-Phospho-RIPK1/RIP-S166 antibody (STJ11103757)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody anti-Phospho-RIPK1/RIP-S166 is suitable for use in Western Blot.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NA


Epitope

NA

Isotype

IgG

Antibody clone number

NA

Vial size

NA

Concentration

NA

Storage

Store in a freezer at-20°C and avoid freeze-thaw cycles.

Handling

NA

Western blot

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-RIPK1/RIP-S166 antibody (STJ11103757) at 1:1000 dilution. NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Western blot analysis of extracts of HT-29 cells, using Phospho-RIPK1/RIP-S166 antibody (STJ11103757) at 1:500 dilution. HT-29 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more