AF-207-NA

antibody from Novus Biologicals

Targeting: IL7 IL-7

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-207-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-7 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human IL-7 in direct ELISAs and Western blots. In Western blots, detects recombinant mouse IL-7 but no cross-reactivity with recombinant rat IL-17.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Interleukin 7 plays a role in T lymphocyte apoptosis inhibition driven by mesenchymal stem cell without favoring proliferation and cytokines secretion.

Normanton M, Alvarenga H, Hamerschlak N, Ribeiro A, Kondo A, Rizzo LV, Marti LC
PloS one 2014;9(9):e106673

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Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.

Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86

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Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.

Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
Journal of immunology (Baltimore, Md. : 1950) 2005 Feb 15;174(4):2376-86

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Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.

Johnson SE, Shah N, Panoskaltsis-Mortari A, LeBien TW
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 1;175(11):7325-31

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Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.

Johnson SE, Shah N, Panoskaltsis-Mortari A, LeBien TW
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 1;175(11):7325-31

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Structure function analysis of interleukin 7: requirement for an aromatic ring at position 143 of helix D.

vanderSpek JC, Sutherland JA, Gill BM, Gorgun G, Foss FM, Murphy JR
Cytokine 2002 Mar 7;17(5):227-33

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Recombinant Human and Mouse IL-7 by Western Blot. Western blot shows 25 ng of Recombinant Human IL-7 (Catalog # 207-IL), Recombinant Mouse IL-7 (Catalog # 407-ML) and Recombinant Rat IL-7 (Catalog # 7857-RL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-7 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-7 and Neutralization by Human IL-7 Antibody. Recombinant Human IL-7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-207-NA). The ND₅₀ is typically 0.02-0.04 µg/mL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody.