AF407

antibody from R&D Systems

Targeting: IL7 IL-7

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF407

Provider

R&D Systems

Product name

Mouse IL-7 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse IL-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant rat IL-7 is observed, and less than 0.1% cross-reactivity with recombinant human IL-7 is observed. Neutralizes the biological activity of recombinant mouse IL-7. It will not neutralize the biological activity of recombinant human IL-7.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q544C8


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Commensal microflora and interferon-gamma promote steady-state interleukin-7 production in vivo.

Shalapour S, Deiser K, Sercan O, Tuckermann J, Minnich K, Willimsky G, Blankenstein T, Hämmerling GJ, Arnold B, Schüler T
European journal of immunology 2010 Sep;40(9):2391-400

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BCL6 is critical for the development of a diverse primary B cell repertoire.

Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jäck HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alborán IM, Melnick AM, Ye BH, Müschen M
The Journal of experimental medicine 2010 Jun 7;207(6):1209-21

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Thymic regulation of autoimmune disease by accelerated differentiation of Foxp3+ regulatory T cells through IL-7 signaling pathway.

Chen X, Fang L, Song S, Guo TB, Liu A, Zhang JZ
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6135-44

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Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.

Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
Blood 2007 May 1;109(9):3706-12

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A recombinant single-chain IL-7/HGFbeta hybrid cytokine induces juxtacrine interactions of the IL-7 and HGF (c-Met) receptors and stimulates the proliferation of CFU-S12, CLPs, and pre-pro-B cells.

Lai L, Zeff RA, Goldschneider I
Blood 2006 Mar 1;107(5):1776-84

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Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.

Johnson SE, Shah N, Panoskaltsis-Mortari A, LeBien TW
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 1;175(11):7325-31

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Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells.

Johnson SE, Shah N, Panoskaltsis-Mortari A, LeBien TW
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 1;175(11):7325-31

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-7 and Neutralization by Mouse IL-7 Antibody. Recombinant Mouse IL-7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF407). The ND₅₀ is typically 0.2-0.4 µg/mL.