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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [2]
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- Product number
- GTX118007 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX118007, RRID:AB_11168085
- Product name
- HEY1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits
Ma L, Mao R, Shen K, Zheng Y, Li Y, Liu J, Ni L
Biochemical and Biophysical Research Communications 2014 July;450(1):353-359
Biochemical and Biophysical Research Communications 2014 July;450(1):353-359
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE GTX118007 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody (GTX118007) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEY1 antibody detects HEY1 protein at nucleus and cytoplasm by immunofluorescent analysis.Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HEY1 protein stained by HEY1 antibody (GTX118007) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEY1 antibody detects HEY1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HEY1 protein stained by HEY1 antibody (GTX118007) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
