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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- 702840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAX1BP1 Recombinant Rabbit Monoclonal Antibody (4H2L18)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody is predicted to react with Monkey, Dog, Horse.
- Antibody clone number
- 4H2L18
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of TAX1BP1 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s16985 and Product # s16984). Western blot analysis (Fig a) was performed using Whole cell extracts from the HeLa knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-TAX1BP1 Recombinant Rabbit Monoclonal Antibody (Product # 702840, 1:200 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to TAX1BP1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), T98G (Lane 3), Hep G2 (Lane 4) and NIH3T3 (Lane 5). The blots were probed with Anti-TAX1BP1 Recombinant Rabbit Monoclonal Antibody (Product # 702840, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 90 kDa band corresponding to TAX1BP1 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous TAX1BP1 using Anti- TAX1BP1 Recombinant Rabbit Monoclonal Antibody (Product # 702840, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000 dilution). Panel a) shows representative cells that were stained for detection and localization of TAX1BP1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of TAX1BP1. Panel e) represents control cells without primary antibody to assess background. The images were captured at 60X magnification.
