12087-2-AP

antibody from Invitrogen Antibodies

Targeting: TSPYL2 CDA1, CINAP, CTCL, DENTT, HRIHFB2216, SE20-4, TSPX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12087-2-AP

Provider

Invitrogen Antibodies

Product name

CDA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: WHRGQEPQA RRHGNQDASH SFFSWFSNHS LPEADRIAEI IKNDLWVNPL RYYLRERGSR IKRKKQEMKK RKTRGRCEVV IMEDAPDYYA VEDIFSEISD IDETIHDIKI SDFMETTDYF ETTDNEITDI NENICDSENP DHNEVPNNET TDNNESADDH ETTDNNESAD DNNENPEDNN KNTDDNEENP NNNENTYGNN FFKGGFWGSH GNNQDSSDSD NEADEASDDE DNDGNEGDNE GSDDDGNEGD NEGSDDDDRD IEYYEKVIED FDKDQADYED VIEIISDESV EEEGIEEGIQ QDEDIYEEGN YEEEGSEDVW EEGEDSDDSD LEDVLQVPNG WANPGKRGKT G (344-693 aa encoded by BC024270)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DU 145 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using TSPYL2 antibody 12087-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 12087-2-AP (CDA1 antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CDA1 (IP:12087-2-AP, 4ug; Detection:12087-2-AP 1:1000) with HEK-293 cells lysate 1000ug.