Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP1-84691 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84691, RRID:AB_11018348
- Product name
- Rabbit Polyclonal PURL Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PURL antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Hypoxia drives the assembly of the multienzyme purinosome complex.
Doigneaux C, Pedley AM, Mistry IN, Papayova M, Benkovic SJ, Tavassoli A
The Journal of biological chemistry 2020 Jul 10;295(28):9551-9566
The Journal of biological chemistry 2020 Jul 10;295(28):9551-9566
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PURL Antibody [NBP1-84691] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PURL Antibody [NBP1-84691] - Analysis using Anti-PFAS antibody NBP1-84691(A) shows similar pattern to independent antibody NBP1-84692 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Staining of human skeletal muscle shows no positivity as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Staining of human testis shows weak to moderate nuclear positivity in a subset of cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Analysis in human lymph node and skeletal muscle tissues. Corresponding PFAS RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Staining of human kidney using Anti-PFAS.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Staining of human kidney, lymph node, skeletal muscle and testis using Anti-PFAS antibody NBP1-84691 (A) shows similar protein distribution across tissues to independent antibody NBP1-84692 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PURL Antibody [NBP1-84691] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal cetner cells.