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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX129122 - Provider product page
- Provider
- GeneTex
- Product name
- CDR2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- CDR2 antibody detects CDR2 protein by western blot analysis.A. 30 £gg HepG2 whole cell lysate/extract10 % SDS-PAGECDR2 antibody (GTX129122) dilution: 1:500
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- GeneTex (provider)
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- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with CDR2 antibody (GTX129122) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- CDR2 antibody detects CDR2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDR2 protein stained by CDR2 antibody (GTX129122) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- CDR2 antibody immunoprecipitates CDR2 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 £gg whole cell lysate/extract of CDR2 protein expressing 293T cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of CDR2 by 2.5 £gg of CDR2 antibody (GTX129122) 10% SDS-PAGE The immunoprecipitated CDR2 protein was detected by CDR2 antibody (GTX129122) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CDR2 antibody detects CDR2 protein on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. CDR2 antibody (GTX129122) dilution: 1:500.
