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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00001039-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001039-M01, RRID:AB_509112
- Product name
- CDR2 monoclonal antibody (M01), clone 4F5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant CDR2.
- Antigen sequence
LTVPESHRKPLKRSSSETILSSLAGSDIVKGHEET
CIRRAKAVKQRGISLLHEVDTQYSALKVKYEELLK
KCQEEQDSLSHKAVQTSRAAAKDLTGVNAQSEPVA
SGWE- Isotype
- IgG
- Antibody clone number
- 4F5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references The onconeural antigen cdr2 is a novel APC/C target that acts in mitosis to regulate c-myc target genes in mammalian tumor cells.
O'Donovan KJ, Diedler J, Couture GC, Fak JJ, Darnell RB
PloS one 2010 Apr 7;5(4):e10045
PloS one 2010 Apr 7;5(4):e10045
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDR2 monoclonal antibody (M01), clone 4F5 Western Blot analysis of CDR2 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDR2 monoclonal antibody (M01), clone 4F5. Western Blot analysis of CDR2 expression in K-562 ( Cat # L009V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CDR2 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CDR2 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
