Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 16490-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16490-1-AP, RRID:AB_2146233
- Product name
- RPS12 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RPS12 antibody (Cat. #16490-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references RNA G-quadruplex structures control ribosomal protein production.
HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis.
On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.
Downregulation of ribosome biogenesis during early forebrain development.
Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.
RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector.
Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts.
Varshney D, Cuesta SM, Herdy B, Abdullah UB, Tannahill D, Balasubramanian S
Scientific reports 2021 Nov 23;11(1):22735
Scientific reports 2021 Nov 23;11(1):22735
HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis.
Katanaev VL, Kryuchkov M, Averkov V, Savitsky M, Nikolaeva K, Klimova N, Khaustov S, Solis GP
Scientific reports 2020 Dec 3;10(1):21013
Scientific reports 2020 Dec 3;10(1):21013
On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.
Shigeoka T, Koppers M, Wong HH, Lin JQ, Cagnetta R, Dwivedy A, de Freitas Nascimento J, van Tartwijk FW, Ströhl F, Cioni JM, Schaeffer J, Carrington M, Kaminski CF, Jung H, Harris WA, Holt CE
Cell reports 2019 Dec 10;29(11):3605-3619.e10
Cell reports 2019 Dec 10;29(11):3605-3619.e10
Downregulation of ribosome biogenesis during early forebrain development.
Chau KF, Shannon ML, Fame RM, Fonseca E, Mullan H, Johnson MB, Sendamarai AK, Springel MW, Laurent B, Lehtinen MK
eLife 2018 May 10;7
eLife 2018 May 10;7
Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.
Chau KF, Springel MW, Broadbelt KG, Park HY, Topal S, Lun MP, Mullan H, Maynard T, Steen H, LaMantia AS, Lehtinen MK
Developmental cell 2015 Dec 21;35(6):789-802
Developmental cell 2015 Dec 21;35(6):789-802
RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector.
Chen D, Zhang R, Shen W, Fu H, Liu S, Sun K, Sun X
International journal of oncology 2013 May;42(5):1763-9
International journal of oncology 2013 May;42(5):1763-9
Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts.
Raines AM, Adam M, Magella B, Meyer SE, Grimes HL, Dey SK, Potter SS
Development (Cambridge, England) 2013 Jul;140(14):2942-52
Development (Cambridge, England) 2013 Jul;140(14):2942-52
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16490-1-AP(RPS12 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RPS12 antibody 16490-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 16490-1-AP(RPS12 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 16490-1-AP(RPS12 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue