PA5-80362

antibody from Invitrogen Antibodies

Targeting: ATP5F1D ATP5D

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80362

Provider

Invitrogen Antibodies

Product name

ATP5D Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human ATP5D.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATP5D in Lane A: HEPG2 Whole Cell Lysate (30 µg), Lane B: Raji Whole Cell Lysate (30 µg), Lane C: MOLT-4 Whole Cell Lysate (30 µg), Lane D: A549 Whole Cell Lysate (30 µg). Samples were probed using a ATP5D Polyclonal Antibody (Product # PA5-80362) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:18 kDa. Observed band size:14 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ATP5D Polyclonal Antibody (Product # PA5-80362) at 1:500 dilution. Lane A: HEPG2 Whole Cell Lysate, Lane B: Raji Whole Cell Lysate, Lane C: MOLT-4 Whole Cell Lysate, Lane D: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 14 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human ATP5D in human lung cancer with ATP5D Polyclonal Antibody (Product # PA5-80362, 1:5,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ATP5D Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg Raji Whole Cell Lysate, Lane C: 0.5 mg A549 Whole Cell Lysate 2 µL with ATP5D Polyclonal Antibody (Product # PA5-80362) and 15 µL of 50 % Protein G agarose. Primary antibody: ATP5D Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 13 kDa. Observed band size: 13 kDa.