AM7610a

antibody from Abgent

Targeting: EPHA5 CEK7, EHK1, Hek7, TYRO4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM7610a

Provider

Abgent

Proper citation

Abgent Cat#AM7610a, RRID:AB_1208744

Product name

EPHA5 Antibody

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Purified Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysate from U-251 MG cell line, using EphA5 Antibody(Cat. #AM7610a). AM7610a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug."

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-EPHA5 Monoclonal Antibody (Cat.#AM7610a) by EPHA5 recombinant protein (Fragment). EPHA5 (Fragment) protein (arrow) was detected using the purified Mab.(1:2000)

Primary Ab dilution

1:2000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human brain tissue reacted with EPHA5 Monoclonal Antibody (Cat.#AM7610a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50