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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- GTX25398 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25398, RRID:AB_380516
- Product name
- EphA5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Gril B, Paranjape AN, Woditschka S, Hua E, Dolan EL, Hanson J, Wu X, Kloc W, Izycka-Swieszewska E, Duchnowska R, Pęksa R, Biernat W, Jassem J, Nayyar N, Brastianos PK, Hall OM, Peer CJ, Figg WD, Pauly GT, Robinson C, Difilippantonio S, Bialecki E, Metellus P, Schneider JP, Steeg PS
Nature communications 2018 Jul 13;9(1):2705
Nature communications 2018 Jul 13;9(1):2705
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of anti-EphA5 C-term Pab (GTX25398) in mouse brain tissue. EphA5 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of anti-EphA5 Antibody (C-term) (GTX25398) in Jurkat cell line lysates (35μg/lane). EPHA5 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary EphA5 antibody (C-term) (GTX25398), which was peroxidase-conjμgated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry.HC = hepatocarcinoma
