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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB541 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat EphA5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat EphA5 in direct ELISAs and Western blots. In direct ELISAs, 5-15% cross reactivity with recombinant mouse (rm) EphA4 and rmEpha8 is observed and no cross-reactivity with recombinant human (rh) EphA1, rmEphA2, -A3, -A6, -A7, recombinant rat EphB1, -B2, or rmEphB3 is observed.
- Reactivity
- Human, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P54757- Isotype
- IgG
- Antibody clone number
- 86731
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ephrin-A5 and EphA5 interaction induces synaptogenesis during early hippocampal development.
Akaneya Y, Sohya K, Kitamura A, Kimura F, Washburn C, Zhou R, Ninan I, Tsumoto T, Ziff EB
PloS one 2010 Aug 31;5(8):e12486
PloS one 2010 Aug 31;5(8):e12486
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EphA5 in U-118-MG Human Cell Line. EphA5 was detected in immersion fixed U-118-MG human glioblastoma/astrocytoma cell line using 10 µg/mL Mouse Anti-Human/Rat EphA5 Monoclonal Antibody (Catalog # MAB541) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of EphA5 in U-118-MG Human Cell Line by Flow Cytometry. U-118-MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human/Rat EphA5 Monoclonal Antibody (Catalog # MAB541, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
