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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- ARG55843 - Provider product page
- Provider
- Arigo
- Product name
- anti-PDIA6 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 144-172 (Center) of Human PDIA6
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of Y79 cell line lysates stained with ARG55843 anti-PDIA6 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of Mouse stomach tissue lysates stained with ARG55843 anti-PDIA6 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of K562, HepG2, HT-1080, Rat C6 cell line and Human liver tissue lysate (from left to right) stained with ARG55843 anti-PDIA6 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HepG2 cells stained with ARG55843 anti-PDIA6 antibody (green) at 1:100 dilution. DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded Human brain tissue stained with ARG55843 anti-PDIA6 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. liver section stained with ARG55843 anti-PDIA6 antibody at 1:100 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. brain section stained with ARG55843 anti-PDIA6 antibody at 1:100 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG55843 anti-PDIA6 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
