H00010130-M04

antibody from Abnova Corporation

Targeting: PDIA6 ERp5, P5, TXNDC7

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010130-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010130-M04, RRID:AB_1679390

Product name

PDIA6 monoclonal antibody (M04), clone 3B4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant PDIA6.

Antigen sequence

MALLVLGLVSCTFFLAVNGLYSSSDDVIELTPSNF
NREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAAT
ALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFG
SNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLG
GRSGGYSSGKQGRSDSSSKKDVIELTDDSFDRNVL
DSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQ
TKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQK
GESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLE
IINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNS
YLEVLLKLADKYKKKMWGWLWTEAGAQSELETALG
IGGFGYPAMAAINARKMKFALLKGSFSEQGINEFL
RELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELP
VEGDIDLSDVELDDLGKDEL


Isotype

IgG

Antibody clone number

3B4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Thioredoxin-interacting protein regulates protein disulfide isomerases and endoplasmic reticulum stress.

Lee S, Min Kim S, Dotimas J, Li L, Feener EP, Baldus S, Myers RB, Chutkow WA, Patwari P, Yoshioka J, Lee RT
EMBO molecular medicine 2014 May 19;6(6):732-43

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PDIA6 expression in transfected 293T cell line by PDIA6 monoclonal antibody (M04), clone 3B4.Lane 1: PDIA6 transfected lysate(48.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PDIA6 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol