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- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX105835 - Provider product page
- Provider
- GeneTex
- Product name
- NDUFS3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis.
Qiao A, Jin X, Pang J, Moskophidis D, Mivechi NF
The Journal of cell biology 2017 Mar 6;216(3):723-741
The Journal of cell biology 2017 Mar 6;216(3):723-741
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- NDUFS3 antibody detects NDUFS3 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX105835) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and NDUFS3 knockout (KO) 293T cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody (GTX105835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NDUFS3 antibody detects NDUFS3 protein at mitochondria by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: NDUFS3 protein stained by NDUFS3 antibody (GTX105835) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. NDUFS3 antibody (GTX105835) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NDUFS3 antibody detects NDUFS3 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. NDUFS3 antibody (GTX105835) dilution: 1:500.
